Sema6B, Sema6C, and Sema6D Expression and Function during Mammalian Retinal Development

Ryota L. Matsuoka, Lu O. Sun, Kei ichi Katayama, Yutaka Yoshida, Alex L Kolodkin

Research output: Contribution to journalArticle

Abstract

In the vertebrate retina, the formation of neural circuits within discrete laminae is critical for the establishment of retinal visual function. Precise formation of retinal circuits requires the coordinated actions of adhesive and repulsive molecules, including repulsive transmembrane semaphorins (Sema6A, Sema5A, and Sema5B). These semaphorins signal through different Plexin A (PlexA) receptors, thereby regulating distinct aspects of retinal circuit assembly. Here, we investigate the physiological roles of three Class 6 transmembrane semaphorins (Sema6B, Sema6C, and Sema6D), previously identified as PlexA receptor ligands in non-retinal tissues, in mammalian retinal development. We performed expression analysis and also phenotypic analyses of mice that carry null mutations in each of genes encoding these proteins using a broad range of inner and outer retinal markers. We find that these Class 6 semaphorins are uniquely expressed throughout postnatal retinal development in specific domains and cell types of the developing retina. However, we do not observe defects in stereotypical lamina-specific neurite stratification of retinal neuron subtypes in Sema6B-/- or Sema6C-/-; Sema6D-/- retinas. These findings indicate these Class 6 transmembrane semaphorins are unlikely to serve as major PlexA receptor ligands for the assembly of murine retinal circuit laminar organization.

Original languageEnglish (US)
Article numbere63207
JournalPLoS One
Volume8
Issue number4
DOIs
StatePublished - Apr 30 2013

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Semaphorins
retina
laminae (animals)
receptors
Retina
Networks (circuits)
neurites
mice
adhesives
Ligands
Retinal Neurons
neurons
vertebrates
Gene encoding
Neurites
Adhesives
Neurons
Vertebrates
Tissue
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sema6B, Sema6C, and Sema6D Expression and Function during Mammalian Retinal Development. / Matsuoka, Ryota L.; Sun, Lu O.; Katayama, Kei ichi; Yoshida, Yutaka; Kolodkin, Alex L.

In: PLoS One, Vol. 8, No. 4, e63207, 30.04.2013.

Research output: Contribution to journalArticle

Matsuoka, Ryota L. ; Sun, Lu O. ; Katayama, Kei ichi ; Yoshida, Yutaka ; Kolodkin, Alex L. / Sema6B, Sema6C, and Sema6D Expression and Function during Mammalian Retinal Development. In: PLoS One. 2013 ; Vol. 8, No. 4.
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