SELF-CONTAINED ENZYMIC MEMBRANE IMMUNOASSAY FOR DETECTION OF ROTAVIRUS ANTIGEN IN CLINICAL SAMPLES

Robert H Yolken, Joseph Eiden, Flora Leister

Research output: Contribution to journalArticle

Abstract

A self-contained enzymic membrane immunoassay (SCEMIA) system has been developed for the detection of viral antigens in clinical samples. The assay system makes use of antiviral antibodies bound to a nylon membrane, a flow-through washing procedure, and a clearly visible endpoint of the enzymic reaction. A SCEMIA system with antibodies against rotavirus detected rotavirus antigen, within 15 min, in all faecal samples from children with gastroenteritis that were positive for antigen in a standard microplate enzyme immunoassay, which took 4 h to complete. In addition, the SCEMIA could detect rotavirus in faecal samples collected from infected individuals both before and after antigen could be detected by a standard immunoassay system. Rotavirus antigen was not detectable in control children who did not have evidence of rotavirus infection. SCEMIA systems are an accurate, rapid, and inexpensive means for the practical diagnosis of viral infections in human beings.

Original languageEnglish (US)
Pages (from-to)1305-1307
Number of pages3
JournalThe Lancet
Volume328
Issue number8519
DOIs
StatePublished - Dec 6 1986

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Rotavirus
Immunoassay
Antigens
Membranes
Rotavirus Infections
Viral Antigens
Antibodies
Nylons
Gastroenteritis
Virus Diseases
Immunoenzyme Techniques
Antiviral Agents

ASJC Scopus subject areas

  • Medicine(all)

Cite this

SELF-CONTAINED ENZYMIC MEMBRANE IMMUNOASSAY FOR DETECTION OF ROTAVIRUS ANTIGEN IN CLINICAL SAMPLES. / Yolken, Robert H; Eiden, Joseph; Leister, Flora.

In: The Lancet, Vol. 328, No. 8519, 06.12.1986, p. 1305-1307.

Research output: Contribution to journalArticle

Yolken, Robert H ; Eiden, Joseph ; Leister, Flora. / SELF-CONTAINED ENZYMIC MEMBRANE IMMUNOASSAY FOR DETECTION OF ROTAVIRUS ANTIGEN IN CLINICAL SAMPLES. In: The Lancet. 1986 ; Vol. 328, No. 8519. pp. 1305-1307.
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