Self-collected versus clinician-collected samples for HSV-2 and HSV-2/HPV screening in HIV-infected and -uninfected women in the Tapajós region, Amazon, Brazil

Luana L.S. Rodrigues, José H. Pilotto, Lyana R.P. Lima, Charlotte A Gaydos, Justin Hardick, Mariza G. Morgado, Katrini G. Martinelli, Vanessa S. de Paula, Alcina F. Nicol

Research output: Contribution to journalArticle

Abstract

The aim of this study was to evaluate the prevalence of herpes simplex virus 2 (HSV-2) and HSV-2/human papillomavirus (HPV) co-infection by self-collected samples compared to clinician-collected samples in human immunodeficiency virus 1 (HIV-1)-infected and -uninfected women from the Tapajós region, Amazon, Brazil. A cross-sectional study was conducted with 439 anal and cervical scrapings and cervico-vaginal self-collected samples obtained from 153 eligible HIV-infected and -uninfected women. Real-time PCR for HSV-2 and nested PCR for HPV detection were performed. A multivariate analysis identified risk factors for HSV-2/HPV co-infection. The anogenital prevalence of HSV-2 was 9.2% (14/153), HPV was 67.3% (103/153) and HSV-2/HPV co-infection was 6.5% (10/153). There was a significant overall agreement (95.5%, 11/133, kappa 0.64, 95% CI 0.38–0.90, p < 0.0001) for HSV-2 detection by the self-collected and clinician-collected samples. HSV-2 genital infection was more prevalent than anal infection in all participants. HIV-infected women had a higher prevalence of HSV-2 and HSV-2/HPV. No woman with a cervical squamous intraepithelial lesion had HSV-2/HPV co-infection. Risk factors for HSV-2/HPV were age ≤25 years (aOR = 10.07) and being single (aOR = 3.79). In general, young and single women are at greater risk for HSV-2/HPV infection. Self-collection can be a useful strategy for the screening of HSV-2 and HPV in limited-resource settings.

Original languageEnglish (US)
JournalInternational Journal of STD and AIDS
DOIs
StateAccepted/In press - Jan 1 2019

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Human Herpesvirus 2
Brazil
HIV
Papillomavirus Infections
Coinfection
Herpes Genitalis
HIV-1
Real-Time Polymerase Chain Reaction

Keywords

  • epidemiology
  • HIV
  • HPV
  • HSV-2
  • self-collected

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Pharmacology (medical)
  • Infectious Diseases

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Self-collected versus clinician-collected samples for HSV-2 and HSV-2/HPV screening in HIV-infected and -uninfected women in the Tapajós region, Amazon, Brazil. / Rodrigues, Luana L.S.; Pilotto, José H.; Lima, Lyana R.P.; Gaydos, Charlotte A; Hardick, Justin; Morgado, Mariza G.; Martinelli, Katrini G.; de Paula, Vanessa S.; Nicol, Alcina F.

In: International Journal of STD and AIDS, 01.01.2019.

Research output: Contribution to journalArticle

Rodrigues, Luana L.S. ; Pilotto, José H. ; Lima, Lyana R.P. ; Gaydos, Charlotte A ; Hardick, Justin ; Morgado, Mariza G. ; Martinelli, Katrini G. ; de Paula, Vanessa S. ; Nicol, Alcina F. / Self-collected versus clinician-collected samples for HSV-2 and HSV-2/HPV screening in HIV-infected and -uninfected women in the Tapajós region, Amazon, Brazil. In: International Journal of STD and AIDS. 2019.
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abstract = "The aim of this study was to evaluate the prevalence of herpes simplex virus 2 (HSV-2) and HSV-2/human papillomavirus (HPV) co-infection by self-collected samples compared to clinician-collected samples in human immunodeficiency virus 1 (HIV-1)-infected and -uninfected women from the Tapaj{\'o}s region, Amazon, Brazil. A cross-sectional study was conducted with 439 anal and cervical scrapings and cervico-vaginal self-collected samples obtained from 153 eligible HIV-infected and -uninfected women. Real-time PCR for HSV-2 and nested PCR for HPV detection were performed. A multivariate analysis identified risk factors for HSV-2/HPV co-infection. The anogenital prevalence of HSV-2 was 9.2{\%} (14/153), HPV was 67.3{\%} (103/153) and HSV-2/HPV co-infection was 6.5{\%} (10/153). There was a significant overall agreement (95.5{\%}, 11/133, kappa 0.64, 95{\%} CI 0.38–0.90, p < 0.0001) for HSV-2 detection by the self-collected and clinician-collected samples. HSV-2 genital infection was more prevalent than anal infection in all participants. HIV-infected women had a higher prevalence of HSV-2 and HSV-2/HPV. No woman with a cervical squamous intraepithelial lesion had HSV-2/HPV co-infection. Risk factors for HSV-2/HPV were age ≤25 years (aOR = 10.07) and being single (aOR = 3.79). In general, young and single women are at greater risk for HSV-2/HPV infection. Self-collection can be a useful strategy for the screening of HSV-2 and HPV in limited-resource settings.",
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AU - Hardick, Justin

AU - Morgado, Mariza G.

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