Self-assembly of Epstein-Barr virus capsids

Brandon W. Henson, Edward M. Perkins, Jonathan E. Cothran, Prashant Desai

Research output: Contribution to journalArticle

Abstract

Epstein-Barr virus (EBV), a member of the Gammaherpesvirus family, primarily infects B lymphocytes and is responsible for a number of lymphoproliferative diseases. The molecular genetics of the assembly pathway and high-resolution structural analysis of the capsid have not been determined for this lymphocryptovirus. As a first step in studying EBV capsid assembly, the baculovirus expression vector (BEV) system was used to express the capsid shell proteins BcLF1 (major capsid protein), BORF1 (triplex protein), BDLF1 (triplex protein), and BFRF3 (small capsid protein); the internal scaffold protein, BdRF1; and the maturational protease (BVRF2). Coinfection of insect cells with the six viruses expressing these proteins resulted in the production of closed capsid structures as judged by electron microscopy and sedimentation methods. Therefore, as shown for other herpesviruses, only six proteins are required for EBV capsid assembly. Furthermore, the small capsid protein of EBV (BFRF3), like that of Kaposi's sarcoma-associated herpesvirus, was found to be required for assembly of a stable structure. Localization of the small capsid protein to nuclear assembly sites required both the major capsid (BcLF1) and scaffold proteins (BdRF1) but not the triplex proteins. Mutational analysis of BFRF3 showed that the N-terminal half (amino acids 1 to 88) of this polypeptide is required and sufficient for capsid assembly. A region spanning amino acids 65 to 88 is required for the concentration of BFRF3 at a subnuclear site and the N-terminal 65 amino acids contain the sequences required for interaction with major capsid protein. These studies have identified the multifunctional role of the gammaherpesvirus small capsid proteins.

Original languageEnglish (US)
Pages (from-to)3877-3890
Number of pages14
JournalJournal of Virology
Volume83
Issue number8
DOIs
StatePublished - Apr 2009

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Human herpesvirus 4
capsid
Capsid
Capsid Proteins
coat proteins
Human Herpesvirus 4
scaffolding proteins
Proteins
Virus Assembly
proteins
Lymphocryptovirus
Human herpesvirus 8
Amino Acids
Human Herpesvirus 8
amino acids
Baculoviridae
Herpesviridae
Coinfection
mixed infection
molecular genetics

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Henson, B. W., Perkins, E. M., Cothran, J. E., & Desai, P. (2009). Self-assembly of Epstein-Barr virus capsids. Journal of Virology, 83(8), 3877-3890. https://doi.org/10.1128/JVI.01733-08

Self-assembly of Epstein-Barr virus capsids. / Henson, Brandon W.; Perkins, Edward M.; Cothran, Jonathan E.; Desai, Prashant.

In: Journal of Virology, Vol. 83, No. 8, 04.2009, p. 3877-3890.

Research output: Contribution to journalArticle

Henson, BW, Perkins, EM, Cothran, JE & Desai, P 2009, 'Self-assembly of Epstein-Barr virus capsids', Journal of Virology, vol. 83, no. 8, pp. 3877-3890. https://doi.org/10.1128/JVI.01733-08
Henson, Brandon W. ; Perkins, Edward M. ; Cothran, Jonathan E. ; Desai, Prashant. / Self-assembly of Epstein-Barr virus capsids. In: Journal of Virology. 2009 ; Vol. 83, No. 8. pp. 3877-3890.
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