Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Joel R. Meyerson, Prashant Rao, Janesh Kumar, Sagar Chittori, Soojay Banerjee, Jason Pierson, Mark L. Mayer, Sriram Subramaniam

Research output: Contribution to journalArticlepeer-review

Abstract

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

Original languageEnglish (US)
Article number7084
JournalScientific Reports
Volume4
DOIs
StatePublished - Nov 18 2014
Externally publishedYes

ASJC Scopus subject areas

  • General
  • Medicine(all)

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