Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster

Research output: Contribution to journalArticle

Abstract

We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.

Original languageEnglish (US)
Pages (from-to)3583-3592
Number of pages10
JournalMolecular and Cellular Biology
Volume5
Issue number12
Publication statusPublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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