Selective thromboxane inhibition after vascular protectant AGI-1067: Results of assessment of lipoprotein profiles (ALPS) biomarkers in vitro and in vivo substudy

Victor Serebruany, Alex Malinin, Fei Hua Qiu, X. C. Xu, Charles Kunsch, Robert Scott

Research output: Contribution to journalArticle

Abstract

Background: Oxidative stress play an important role triggering platelet/ endothelial activation. AGI-1067 is a novel, phenolic antioxidant, and vascular protectant which dose-dependently inhibits PEA biomarkers in vitro. Whether treatment with AGI-1067 alters platelets in vivo is not known. We serially assessed release of established PEA biomarkers in subjects treated with AGI-1067 versus placebo in the frame of Assessment of Lipoprotein Profiles Randomized Trial (ALPS). Methods: Healthy subjects (18-65 years) with multiple risk factors for coronary artery disease were randomized 1:1 to receive 300 mg AGI-1067 (n = 112) or matching placebo (n = 117) daily for 12 weeks. Anticoagulants, aspirin, NSAIDS, and COX inhibitors were not permitted in this study. Plasma samples were collected at baseline, and at week 12 after randomization. Platelet factor 4 (PF4), β-thromboglobulin (βTG), P-selectin, thromboxane (TxB2), and prostacyclin (6-keto-PGF1a) were measured by ELISA. Results: Treatment with AGI-1067 was associated with a highly significant reduction of TxB2 release (P <0.0001) when compared to the placebo. There were no differences in PF4, βTG, P-selectin, and 6-keto-PGF1a between and within groups. AGI-1067 also inhibits TxB2 release from calcium ionophore (A23187)-stimulated human platelets with the IC50 equals 1 μM; but does not interfere with 6-keto-PGF1α release in either A23187-, or TXA2-stimulated human aortic endothelial cells. Conclusion: AGI-1067 selectively reduces TxB2 production from stimulated platelets, and diminishes plasma TxB2 levels in ALPS participants. These data support earlier in vitro, and pilot ex vivo experiments suggesting antiplatelet properties of AGI-1067. Lack of 6-keto-PGF1a down regulation may represent an attractive advantage of AGI-1067 over currently available antiplatelet regimens.

Original languageEnglish (US)
Pages (from-to)438-446
Number of pages9
JournalJournal of Thrombosis and Thrombolysis
Volume27
Issue number4
DOIs
StatePublished - 2009

Keywords

  • AGI-1067
  • Clinical trial
  • Platelets
  • Thromboxane
  • Vascular protectant

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Selective thromboxane inhibition after vascular protectant AGI-1067: Results of assessment of lipoprotein profiles (ALPS) biomarkers in vitro and in vivo substudy'. Together they form a unique fingerprint.

  • Cite this