Selective synthesis of octadeuterated (±)-5-HETE for use in GC-MS quantitation of 5-HETE

W. C. Hubbard, M. A. Phillips, D. F. Taber

Research output: Contribution to journalArticle

Abstract

5(S)-hydroxy-6 trans-8-11,14 cis-eicosatetraenoic acid (5-HETE) is the major product of arachidonic acid metabolism via the 5-lipoxygenase pathway. A limiting factor in the quatitation of 5-HETE by GC-MS analysis is the availability of a stable isotope analog for use as an internal standard. In this report, we detail procedures for selective chemical synthesis of multimilligram quantities of octadeuterated (±)-5-HETE from octadeuterated arachidonic acid. The octadeuterated (±)-5-HETE is suitable for use as an internal standard for GC-MS quantitation of 5-HETE. Preparation of the octadeuterated analog of 5-HETE can be readily performed in most laboratory settings.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalProstaglandins
Volume23
Issue number1
DOIs
StatePublished - Jan 1982

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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