Selective Stimulation of Nonhistone Chromatin Protein Synthesis in Lymphoid Cells by Phytohemagglutinin

Ronald Levy, Shoshana Levy, Steven A. Rosenberg, Robert T. Simpson

Research output: Contribution to journalArticle

Abstract

The chromatin proteins synthesized by phyto-hemagglutinin-stimulated lymphocytes have been investigated using a double isotope labeling approach. Lymphocyte chromatin was dissociated in 0.4 M guanidine-HCl, 6 M urea, 0.1% β-mercaptoethanol, and 0.1 M sodium phosphate buffer at pH 7.0, which solubilized >90% of the total chromatin proteins. The histones and nonhistones were then separated from one another by ion exchange chromatography and analyzed by polyacrylamide gel electrophoresis. The nonhistone chromatin proteins accounted for less than 15% of the total protein in lymphocyte chromatin but they contained >70% of the [3H]leucine incorporated into the chromatin protein during a 2-hr pulse. When lymphocytes were stimulated by phytohemagglutinin, there was a prompt increase in the synthesis of all the nonhistone proteins, but not of histones. Among the nonhistones, several specific proteins were preferentially synthesized during the activation of the cell by phytohemagglutinin.

Original languageEnglish (US)
Pages (from-to)224-228
Number of pages5
JournalBiochemistry
Volume12
Issue number2
DOIs
StatePublished - Jan 1 1973

ASJC Scopus subject areas

  • Biochemistry

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