Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes

M. L. Elks; P. A. Watkins; V. C. Manganiello; J. Moss; E. Hewlett; M. Vaughan

doi:10.1016/0006-291X(83)90565-X

Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes

M. L. Elks, P. A. Watkins, V. C. Manganiello, J. Moss, E. Hewlett, M. Vaughan

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Incubation of 3T3-L1 adipocytes with insulin or isoproterenol for 10 min increased particulate "low K_m" cAMP phosphodiesterase activity by 42% and 50%, respectively. Pertussis toxin catalyzed the [³²P]-ADP ribosylation of a 41,000 dalton protein in adipocyte particulate fractions; prior incubation of adipocytes with toxin markedly reduced incorporation of radiolabel. Exposure of adipocytes to pertussis toxin (0.3 μg, 18 hr) increased glycerol production and inhibited activation of cAMP phosphodiesterase by insulin, but not by isoproterenol. These results suggest that pertussis toxin can interfere with receptor-mediated processes that stimulate cAMP hydrolysis as well as those that inhibit cAMP formation.

Original language	English (US)
Pages (from-to)	593-598
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	116
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(83)90565-X
State	Published - Oct 31 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(83)90565-X

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title = "Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes",

abstract = "Incubation of 3T3-L1 adipocytes with insulin or isoproterenol for 10 min increased particulate {"}low Km{"} cAMP phosphodiesterase activity by 42% and 50%, respectively. Pertussis toxin catalyzed the [32P]-ADP ribosylation of a 41,000 dalton protein in adipocyte particulate fractions; prior incubation of adipocytes with toxin markedly reduced incorporation of radiolabel. Exposure of adipocytes to pertussis toxin (0.3 μg, 18 hr) increased glycerol production and inhibited activation of cAMP phosphodiesterase by insulin, but not by isoproterenol. These results suggest that pertussis toxin can interfere with receptor-mediated processes that stimulate cAMP hydrolysis as well as those that inhibit cAMP formation.",

author = "Elks, {M. L.} and Watkins, {P. A.} and Manganiello, {V. C.} and J. Moss and E. Hewlett and M. Vaughan",

year = "1983",

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T1 - Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes

AU - Elks, M. L.

AU - Watkins, P. A.

AU - Manganiello, V. C.

AU - Moss, J.

AU - Hewlett, E.

AU - Vaughan, M.

PY - 1983/10/31

Y1 - 1983/10/31

N2 - Incubation of 3T3-L1 adipocytes with insulin or isoproterenol for 10 min increased particulate "low Km" cAMP phosphodiesterase activity by 42% and 50%, respectively. Pertussis toxin catalyzed the [32P]-ADP ribosylation of a 41,000 dalton protein in adipocyte particulate fractions; prior incubation of adipocytes with toxin markedly reduced incorporation of radiolabel. Exposure of adipocytes to pertussis toxin (0.3 μg, 18 hr) increased glycerol production and inhibited activation of cAMP phosphodiesterase by insulin, but not by isoproterenol. These results suggest that pertussis toxin can interfere with receptor-mediated processes that stimulate cAMP hydrolysis as well as those that inhibit cAMP formation.

AB - Incubation of 3T3-L1 adipocytes with insulin or isoproterenol for 10 min increased particulate "low Km" cAMP phosphodiesterase activity by 42% and 50%, respectively. Pertussis toxin catalyzed the [32P]-ADP ribosylation of a 41,000 dalton protein in adipocyte particulate fractions; prior incubation of adipocytes with toxin markedly reduced incorporation of radiolabel. Exposure of adipocytes to pertussis toxin (0.3 μg, 18 hr) increased glycerol production and inhibited activation of cAMP phosphodiesterase by insulin, but not by isoproterenol. These results suggest that pertussis toxin can interfere with receptor-mediated processes that stimulate cAMP hydrolysis as well as those that inhibit cAMP formation.

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Selective regulation by pertussis toxin of insulin-induced activation of particulate cAMP phosphodiesterase activity in 3T3-L1 adipocytes

Abstract

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