Selective NR2B NMDA receptor antagonists are protective against staurosporine-induced apoptosis

Anthony J. Williams; Jitendra R. Dave; X. May Lu; Geoff Ling; Frank C. Tortella

doi:10.1016/S0014-2999(02)02327-0

Selective NR2B NMDA receptor antagonists are protective against staurosporine-induced apoptosis

Anthony J. Williams, Jitendra R. Dave, X. May Lu, Geoff Ling, Frank C. Tortella

School of Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Staurosporine-induced apoptosis was associated with a 20% cellular survival rate in primary rat forebrain cultures. Treatment with the NR2B subunit-selective NMDA receptor antagonist conantokin-G (0.1-1 μM) increased the survival rate up to 78%. No protection was provided by the nonselective NMDA receptor antagonist dizocilpine (0.01-10 μM) but 34-64% cellular survival was provided by ifenprodil (0.01-10 μM), another NR2B subunit-selective antagonist. These results suggest a novel anti-apoptotic mechanism linked to the NR2B receptor subunit.

Original language	English (US)
Pages (from-to)	135-136
Number of pages	2
Journal	European Journal of Pharmacology
Volume	452
Issue number	1
DOIs	https://doi.org/10.1016/S0014-2999(02)02327-0
State	Published - Sep 27 2002

Keywords

Conantokin-G
Neuronal culture
Staurosporine

ASJC Scopus subject areas

Pharmacology

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10.1016/S0014-2999(02)02327-0

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title = "Selective NR2B NMDA receptor antagonists are protective against staurosporine-induced apoptosis",

abstract = "Staurosporine-induced apoptosis was associated with a 20% cellular survival rate in primary rat forebrain cultures. Treatment with the NR2B subunit-selective NMDA receptor antagonist conantokin-G (0.1-1 μM) increased the survival rate up to 78%. No protection was provided by the nonselective NMDA receptor antagonist dizocilpine (0.01-10 μM) but 34-64% cellular survival was provided by ifenprodil (0.01-10 μM), another NR2B subunit-selective antagonist. These results suggest a novel anti-apoptotic mechanism linked to the NR2B receptor subunit.",

keywords = "Conantokin-G, Neuronal culture, Staurosporine",

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AU - Williams, Anthony J.

AU - Dave, Jitendra R.

AU - Lu, X. May

AU - Ling, Geoff

AU - Tortella, Frank C.

PY - 2002/9/27

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N2 - Staurosporine-induced apoptosis was associated with a 20% cellular survival rate in primary rat forebrain cultures. Treatment with the NR2B subunit-selective NMDA receptor antagonist conantokin-G (0.1-1 μM) increased the survival rate up to 78%. No protection was provided by the nonselective NMDA receptor antagonist dizocilpine (0.01-10 μM) but 34-64% cellular survival was provided by ifenprodil (0.01-10 μM), another NR2B subunit-selective antagonist. These results suggest a novel anti-apoptotic mechanism linked to the NR2B receptor subunit.

AB - Staurosporine-induced apoptosis was associated with a 20% cellular survival rate in primary rat forebrain cultures. Treatment with the NR2B subunit-selective NMDA receptor antagonist conantokin-G (0.1-1 μM) increased the survival rate up to 78%. No protection was provided by the nonselective NMDA receptor antagonist dizocilpine (0.01-10 μM) but 34-64% cellular survival was provided by ifenprodil (0.01-10 μM), another NR2B subunit-selective antagonist. These results suggest a novel anti-apoptotic mechanism linked to the NR2B receptor subunit.

KW - Conantokin-G

KW - Neuronal culture

KW - Staurosporine

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Selective NR2B NMDA receptor antagonists are protective against staurosporine-induced apoptosis

Abstract

Keywords

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