Selective modulation of protein kinase C-θ during T-cell activation

Colin R.F. Monks, Hannah Kupfer, Idan Tamir, Avlin Barlow, Abraham Kupfer

Research output: Contribution to journalArticle

Abstract

EVERY cell contains many families of protein kinases, and may express several structurally related yet genetically distinct kinases of each family. The activity of the serine/threonine protein kinase C (PKC) enzymes has long been implicated in T-cell activation, but it is not known which members of the PKC family regulate the T-cell response to foreign antigens. The activation of T cells by antigen-presenting cells (APCs) is spatially restricted to their site of contact, where receptors on the T cells engage their counter-receptors on the APCs. We used this localized engagement to identify, at the single-cell level, intracellular proteins involved in the activation process. By digital immunofluorescence microscopy, we localized six isoforms of PKC in antigen-specific T-cell clones activated by APCs. Surprisingly, only PKC-θ translocated to the site of cell contact. Accordingly, in vitro kinase activity assays of PKC immunoprecipitates from the conjugates of T cells and APCs showed a selective increase in the activity of PKC-θ, indicating that the translocated enzyme is active. Several modes of partial T-cell activation that failed to cause PKC-θ translocation also failed to cause T-cell proliferation, further suggesting the involvement of PKC-θ in T-cell activation.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalNature
Volume385
Issue number6611
DOIs
StatePublished - Jan 2 1997

ASJC Scopus subject areas

  • General

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    Monks, C. R. F., Kupfer, H., Tamir, I., Barlow, A., & Kupfer, A. (1997). Selective modulation of protein kinase C-θ during T-cell activation. Nature, 385(6611), 83-86. https://doi.org/10.1038/385083a0