Selective labeling of α-noradrenergic receptors in rat brain with [3H] dihydroergokryptine

David A. Greenberg, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Using concentrations of [3H] dihydroergokryptine between 0.1 and 5 nM, saturable binding can be demonstrated in rat cerebral cortical membranes with a dissociation constant (KD) of about 0.8 nM. α-Noradrenergic agonists and antagonists compete for the sites labeled by these low concentrations of [3H] dihydroergokryptine with relative potencies characteristics of classical α-noradrenergic receptors. The very low potency of serotonin in competing for these binding sites indicates that, in contrast to findings with higher concentrations of [3H] DHE, low concentrations do not label serotonin receptors. Moreover, the low potency of dopamine in competing for [3H] dihydroergokryptine binding in both striatal and cortical membranes indicates that no detectable portion of binding is associated with postsynaptic dopamine receptors.

Original languageEnglish (US)
Pages (from-to)927-931
Number of pages5
JournalLife Sciences
Volume20
Issue number6
DOIs
StatePublished - Mar 15 1977

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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