Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

F. Sieber; J. L. Spivak; A. M. Sutcliffe

doi:10.1073/pnas.81.23.7584

Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

F. Sieber, J. L. Spivak, A. M. Sutcliffe

School of Medicine

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

Original language	English (US)
Pages (from-to)	7584-7587
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	81
Issue number	23 I
DOIs	https://doi.org/10.1073/pnas.81.23.7584
State	Published - 1984

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.81.23.7584

Cite this

@article{8afb335cddc743b389c5fe8a567be60e,

title = "Selective killing of leukemic cells by merocyanine 540-mediated photosensitization",

abstract = "In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.",

author = "F. Sieber and Spivak, {J. L.} and Sutcliffe, {A. M.}",

year = "1984",

doi = "10.1073/pnas.81.23.7584",

language = "English (US)",

volume = "81",

pages = "7584--7587",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "23 I",

}

TY - JOUR

T1 - Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

AU - Sieber, F.

AU - Spivak, J. L.

AU - Sutcliffe, A. M.

PY - 1984

Y1 - 1984

N2 - In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

AB - In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

UR - http://www.scopus.com/inward/record.url?scp=0344041416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344041416&partnerID=8YFLogxK

U2 - 10.1073/pnas.81.23.7584

DO - 10.1073/pnas.81.23.7584

M3 - Article

C2 - 6594700

AN - SCOPUS:0344041416

SN - 0027-8424

VL - 81

SP - 7584

EP - 7587

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 23 I

ER -

Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this