Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

F. Sieber, Jerry L Spivak, A. M. Sutcliffe

Research output: Contribution to journalArticle

Abstract

In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

Original languageEnglish (US)
Pages (from-to)7584-7587
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number23 I
StatePublished - 1984

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Photosensitivity Disorders
Leukemia L1210
Pluripotent Stem Cells
Hematopoietic Stem Cells
Fluorescent Dyes
Bone Marrow Cells
Bone Marrow
Transplants
Light
Neoplasms
In Vitro Techniques
merocyanine dye

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. / Sieber, F.; Spivak, Jerry L; Sutcliffe, A. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 23 I, 1984, p. 7584-7587.

Research output: Contribution to journalArticle

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