Selective inhibition of interleukin-4 gene expression in human T cells by aspirin

Antonella Cianferoni, John Thomas Schroeder, Jean Kim, John W. Schmidt, Lawrence M. Lichtenstein, Steve N. Georas, Vincenzo Casolaro

Research output: Contribution to journalArticle

Abstract

Previous studies indicated that aspirin (acetylsalicylic acid [ASA]) can have profound immunomodulatory effects by regulating cytokine gene expression in several types of cells. This study is the first in which concentrations of ASA in the therapeutic range were found to significantly reduce interleukin (IL)-4 secretion and RNA expression in freshly isolated and mitogen-primed human CD4+ T cells. In contrast, ASA did not affect IL-13, interferon-γ, and IL-2 expression. ASA inhibited IL-4, but not IL-2, promoter-driven chloramphenicol acetyltransferase expression in transiently transfected Jurkat T cells. The structurally unrelated non-steroidal anti-inflammatory drugs indomethacin and flurbiprofen did not affect cytokine gene expression in T cells, whereas the weak cyclo-oxygenase inhibitor salicylic acid was at least as effective as ASA in inhibiting IL-4 expression and promoter activity. The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-κB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-κB-binding P1 element. It is concluded that anti-inflammatory salicylates, by means of a previously unrecognized mechanism of action, can influence the nature of adaptive immune responses by selectively inhibiting the expression of IL-4, a critical effector of these responses, in CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)1742-1749
Number of pages8
JournalBlood
Volume97
Issue number6
DOIs
StatePublished - Mar 15 2001

Fingerprint

T-cells
Gene expression
Interleukin-4
Aspirin
T-Lymphocytes
Gene Expression
Salicylates
Interleukin-2
Anti-Inflammatory Agents
Cytokines
NFATC Transcription Factors
Flurbiprofen
TCF Transcription Factors
Chloramphenicol O-Acetyltransferase
Jurkat Cells
Interleukin-13
Salicylic Acid
Cyclooxygenase Inhibitors
Adaptive Immunity
Transcription

ASJC Scopus subject areas

  • Hematology

Cite this

Cianferoni, A., Schroeder, J. T., Kim, J., Schmidt, J. W., Lichtenstein, L. M., Georas, S. N., & Casolaro, V. (2001). Selective inhibition of interleukin-4 gene expression in human T cells by aspirin. Blood, 97(6), 1742-1749. https://doi.org/10.1182/blood.V97.6.1742

Selective inhibition of interleukin-4 gene expression in human T cells by aspirin. / Cianferoni, Antonella; Schroeder, John Thomas; Kim, Jean; Schmidt, John W.; Lichtenstein, Lawrence M.; Georas, Steve N.; Casolaro, Vincenzo.

In: Blood, Vol. 97, No. 6, 15.03.2001, p. 1742-1749.

Research output: Contribution to journalArticle

Cianferoni, A, Schroeder, JT, Kim, J, Schmidt, JW, Lichtenstein, LM, Georas, SN & Casolaro, V 2001, 'Selective inhibition of interleukin-4 gene expression in human T cells by aspirin', Blood, vol. 97, no. 6, pp. 1742-1749. https://doi.org/10.1182/blood.V97.6.1742
Cianferoni, Antonella ; Schroeder, John Thomas ; Kim, Jean ; Schmidt, John W. ; Lichtenstein, Lawrence M. ; Georas, Steve N. ; Casolaro, Vincenzo. / Selective inhibition of interleukin-4 gene expression in human T cells by aspirin. In: Blood. 2001 ; Vol. 97, No. 6. pp. 1742-1749.
@article{60234b2a54bc4b14b96b76b83c9befbd,
title = "Selective inhibition of interleukin-4 gene expression in human T cells by aspirin",
abstract = "Previous studies indicated that aspirin (acetylsalicylic acid [ASA]) can have profound immunomodulatory effects by regulating cytokine gene expression in several types of cells. This study is the first in which concentrations of ASA in the therapeutic range were found to significantly reduce interleukin (IL)-4 secretion and RNA expression in freshly isolated and mitogen-primed human CD4+ T cells. In contrast, ASA did not affect IL-13, interferon-γ, and IL-2 expression. ASA inhibited IL-4, but not IL-2, promoter-driven chloramphenicol acetyltransferase expression in transiently transfected Jurkat T cells. The structurally unrelated non-steroidal anti-inflammatory drugs indomethacin and flurbiprofen did not affect cytokine gene expression in T cells, whereas the weak cyclo-oxygenase inhibitor salicylic acid was at least as effective as ASA in inhibiting IL-4 expression and promoter activity. The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-κB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-κB-binding P1 element. It is concluded that anti-inflammatory salicylates, by means of a previously unrecognized mechanism of action, can influence the nature of adaptive immune responses by selectively inhibiting the expression of IL-4, a critical effector of these responses, in CD4+ T cells.",
author = "Antonella Cianferoni and Schroeder, {John Thomas} and Jean Kim and Schmidt, {John W.} and Lichtenstein, {Lawrence M.} and Georas, {Steve N.} and Vincenzo Casolaro",
year = "2001",
month = "3",
day = "15",
doi = "10.1182/blood.V97.6.1742",
language = "English (US)",
volume = "97",
pages = "1742--1749",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - Selective inhibition of interleukin-4 gene expression in human T cells by aspirin

AU - Cianferoni, Antonella

AU - Schroeder, John Thomas

AU - Kim, Jean

AU - Schmidt, John W.

AU - Lichtenstein, Lawrence M.

AU - Georas, Steve N.

AU - Casolaro, Vincenzo

PY - 2001/3/15

Y1 - 2001/3/15

N2 - Previous studies indicated that aspirin (acetylsalicylic acid [ASA]) can have profound immunomodulatory effects by regulating cytokine gene expression in several types of cells. This study is the first in which concentrations of ASA in the therapeutic range were found to significantly reduce interleukin (IL)-4 secretion and RNA expression in freshly isolated and mitogen-primed human CD4+ T cells. In contrast, ASA did not affect IL-13, interferon-γ, and IL-2 expression. ASA inhibited IL-4, but not IL-2, promoter-driven chloramphenicol acetyltransferase expression in transiently transfected Jurkat T cells. The structurally unrelated non-steroidal anti-inflammatory drugs indomethacin and flurbiprofen did not affect cytokine gene expression in T cells, whereas the weak cyclo-oxygenase inhibitor salicylic acid was at least as effective as ASA in inhibiting IL-4 expression and promoter activity. The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-κB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-κB-binding P1 element. It is concluded that anti-inflammatory salicylates, by means of a previously unrecognized mechanism of action, can influence the nature of adaptive immune responses by selectively inhibiting the expression of IL-4, a critical effector of these responses, in CD4+ T cells.

AB - Previous studies indicated that aspirin (acetylsalicylic acid [ASA]) can have profound immunomodulatory effects by regulating cytokine gene expression in several types of cells. This study is the first in which concentrations of ASA in the therapeutic range were found to significantly reduce interleukin (IL)-4 secretion and RNA expression in freshly isolated and mitogen-primed human CD4+ T cells. In contrast, ASA did not affect IL-13, interferon-γ, and IL-2 expression. ASA inhibited IL-4, but not IL-2, promoter-driven chloramphenicol acetyltransferase expression in transiently transfected Jurkat T cells. The structurally unrelated non-steroidal anti-inflammatory drugs indomethacin and flurbiprofen did not affect cytokine gene expression in T cells, whereas the weak cyclo-oxygenase inhibitor salicylic acid was at least as effective as ASA in inhibiting IL-4 expression and promoter activity. The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-κB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-κB-binding P1 element. It is concluded that anti-inflammatory salicylates, by means of a previously unrecognized mechanism of action, can influence the nature of adaptive immune responses by selectively inhibiting the expression of IL-4, a critical effector of these responses, in CD4+ T cells.

UR - http://www.scopus.com/inward/record.url?scp=0035869391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035869391&partnerID=8YFLogxK

U2 - 10.1182/blood.V97.6.1742

DO - 10.1182/blood.V97.6.1742

M3 - Article

C2 - 11238116

AN - SCOPUS:0035869391

VL - 97

SP - 1742

EP - 1749

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -