Selective increases in antibody isotypes and immunoglobulin G subclass responses to secreted antigens in tuberculosis patients and healthy household contacts of the patients

R. Hussain, G. Dawood, N. Abrar, Z. Toossi, A. Minai, M. Dojki, J. J. Ellner

Research output: Contribution to journalArticle

Abstract

Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity, with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P <0.001) and IgG1 (P <0.001) antibodies, Pulmonary patients also showed a significant difference with IgM (P <0.01) and IgE (P <0.05) antibodies, HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P <0.05) in patients compared with EC. A strong correlation (rho = 0.254; P <0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.

Original languageEnglish (US)
Pages (from-to)726-732
Number of pages7
JournalClinical and Diagnostic Laboratory Immunology
Volume2
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Tuberculosis
Immunoglobulin G
Antigens
Antibodies
Immunoglobulin M
Mycobacterium tuberculosis
Tissue Donors
Immunoglobulin E
Antibody Formation
Lymph Nodes
Lung
Immunosorbents
Skin Tests
Pulmonary Tuberculosis
Disease Progression
Assays
Skin
Chemical activation
Enzyme-Linked Immunosorbent Assay
Derivatives

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Selective increases in antibody isotypes and immunoglobulin G subclass responses to secreted antigens in tuberculosis patients and healthy household contacts of the patients. / Hussain, R.; Dawood, G.; Abrar, N.; Toossi, Z.; Minai, A.; Dojki, M.; Ellner, J. J.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 2, No. 6, 1995, p. 726-732.

Research output: Contribution to journalArticle

@article{ea4cd62a2aca446cbfa63203723eee38,
title = "Selective increases in antibody isotypes and immunoglobulin G subclass responses to secreted antigens in tuberculosis patients and healthy household contacts of the patients",
abstract = "Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity, with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P <0.001) and IgG1 (P <0.001) antibodies, Pulmonary patients also showed a significant difference with IgM (P <0.01) and IgE (P <0.05) antibodies, HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P <0.05) in patients compared with EC. A strong correlation (rho = 0.254; P <0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.",
author = "R. Hussain and G. Dawood and N. Abrar and Z. Toossi and A. Minai and M. Dojki and Ellner, {J. J.}",
year = "1995",
language = "English (US)",
volume = "2",
pages = "726--732",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Selective increases in antibody isotypes and immunoglobulin G subclass responses to secreted antigens in tuberculosis patients and healthy household contacts of the patients

AU - Hussain, R.

AU - Dawood, G.

AU - Abrar, N.

AU - Toossi, Z.

AU - Minai, A.

AU - Dojki, M.

AU - Ellner, J. J.

PY - 1995

Y1 - 1995

N2 - Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity, with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P <0.001) and IgG1 (P <0.001) antibodies, Pulmonary patients also showed a significant difference with IgM (P <0.01) and IgE (P <0.05) antibodies, HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P <0.05) in patients compared with EC. A strong correlation (rho = 0.254; P <0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.

AB - Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity, with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P <0.001) and IgG1 (P <0.001) antibodies, Pulmonary patients also showed a significant difference with IgM (P <0.01) and IgE (P <0.05) antibodies, HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P <0.05) in patients compared with EC. A strong correlation (rho = 0.254; P <0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.

UR - http://www.scopus.com/inward/record.url?scp=0028845408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028845408&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 726

EP - 732

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 6

ER -