Selective expression of Narp in primary nociceptive neurons: Role in microglia/macrophage activation following nerve injury

Research output: Contribution to journalArticle

Abstract

Neuronal activity regulated pentraxin (Narp) is a secreted protein implicated in regulating synaptic plasticity via its association with the extracellular surface of AMPA receptors. We found robust Narp immunostaining in dorsal root ganglia (DRG) that is largely restricted to small diameter neurons, and in the superficial layers of the dorsal horn of the spinal cord. In double staining studies of DRG, we found that Narp is expressed in both IB4- and CGRP-positive neurons, markers of distinct populations of nociceptive neurons. Although a panel of standard pain behavioral assays were unaffected by Narp deletion, we found that Narp knockout mice displayed an exaggerated microglia/macrophage response in the dorsal horn of the spinal cord to sciatic nerve transection 3. days after surgery compared with wild type mice. As other members of the pentraxin family have been implicated in regulating innate immunity, these findings suggest that Narp, and perhaps other neuronal pentraxins, also regulate inflammation in the nervous system.

Original languageEnglish (US)
Pages (from-to)86-95
Number of pages10
JournalJournal of Neuroimmunology
Volume274
Issue number1-2
DOIs
StatePublished - 2014

Fingerprint

Nociceptors
Macrophage Activation
Spinal Ganglia
Microglia
Neurons
Neuronal Plasticity
AMPA Receptors
Wounds and Injuries
Sciatic Nerve
Ambulatory Surgical Procedures
Innate Immunity
Knockout Mice
Nervous System
Macrophages
Staining and Labeling
Inflammation
Pain
Population
Proteins
Spinal Cord Dorsal Horn

Keywords

  • AMPA
  • CGRP
  • Dorsal root ganglion
  • Inflammation
  • Pain
  • Pentraxin

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

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title = "Selective expression of Narp in primary nociceptive neurons: Role in microglia/macrophage activation following nerve injury",
abstract = "Neuronal activity regulated pentraxin (Narp) is a secreted protein implicated in regulating synaptic plasticity via its association with the extracellular surface of AMPA receptors. We found robust Narp immunostaining in dorsal root ganglia (DRG) that is largely restricted to small diameter neurons, and in the superficial layers of the dorsal horn of the spinal cord. In double staining studies of DRG, we found that Narp is expressed in both IB4- and CGRP-positive neurons, markers of distinct populations of nociceptive neurons. Although a panel of standard pain behavioral assays were unaffected by Narp deletion, we found that Narp knockout mice displayed an exaggerated microglia/macrophage response in the dorsal horn of the spinal cord to sciatic nerve transection 3. days after surgery compared with wild type mice. As other members of the pentraxin family have been implicated in regulating innate immunity, these findings suggest that Narp, and perhaps other neuronal pentraxins, also regulate inflammation in the nervous system.",
keywords = "AMPA, CGRP, Dorsal root ganglion, Inflammation, Pain, Pentraxin",
author = "M. Miskimon and S. Han and Lee, {J. J.} and Matthias Ringkamp and Wilson, {Mary Ann Ann} and Petralia, {R. S.} and Xinzhong Dong and Worley, {Paul F} and Baraban, {Jay M} and Reti, {Irving M}",
year = "2014",
doi = "10.1016/j.jneuroim.2014.06.016",
language = "English (US)",
volume = "274",
pages = "86--95",
journal = "Journal of Neuroimmunology",
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T2 - Role in microglia/macrophage activation following nerve injury

AU - Miskimon, M.

AU - Han, S.

AU - Lee, J. J.

AU - Ringkamp, Matthias

AU - Wilson, Mary Ann Ann

AU - Petralia, R. S.

AU - Dong, Xinzhong

AU - Worley, Paul F

AU - Baraban, Jay M

AU - Reti, Irving M

PY - 2014

Y1 - 2014

N2 - Neuronal activity regulated pentraxin (Narp) is a secreted protein implicated in regulating synaptic plasticity via its association with the extracellular surface of AMPA receptors. We found robust Narp immunostaining in dorsal root ganglia (DRG) that is largely restricted to small diameter neurons, and in the superficial layers of the dorsal horn of the spinal cord. In double staining studies of DRG, we found that Narp is expressed in both IB4- and CGRP-positive neurons, markers of distinct populations of nociceptive neurons. Although a panel of standard pain behavioral assays were unaffected by Narp deletion, we found that Narp knockout mice displayed an exaggerated microglia/macrophage response in the dorsal horn of the spinal cord to sciatic nerve transection 3. days after surgery compared with wild type mice. As other members of the pentraxin family have been implicated in regulating innate immunity, these findings suggest that Narp, and perhaps other neuronal pentraxins, also regulate inflammation in the nervous system.

AB - Neuronal activity regulated pentraxin (Narp) is a secreted protein implicated in regulating synaptic plasticity via its association with the extracellular surface of AMPA receptors. We found robust Narp immunostaining in dorsal root ganglia (DRG) that is largely restricted to small diameter neurons, and in the superficial layers of the dorsal horn of the spinal cord. In double staining studies of DRG, we found that Narp is expressed in both IB4- and CGRP-positive neurons, markers of distinct populations of nociceptive neurons. Although a panel of standard pain behavioral assays were unaffected by Narp deletion, we found that Narp knockout mice displayed an exaggerated microglia/macrophage response in the dorsal horn of the spinal cord to sciatic nerve transection 3. days after surgery compared with wild type mice. As other members of the pentraxin family have been implicated in regulating innate immunity, these findings suggest that Narp, and perhaps other neuronal pentraxins, also regulate inflammation in the nervous system.

KW - AMPA

KW - CGRP

KW - Dorsal root ganglion

KW - Inflammation

KW - Pain

KW - Pentraxin

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