Selective expression of adhesion molecules on human brain microvascular endothelial cells

Research output: Contribution to journalArticlepeer-review

Abstract

Human microvascular endothelial cells were isolated from children's brain and examined for their morphological characteristics and upregulation of cell adhesion molecules in response to TNFα. Our human brain microvascular endothelial cells (HBMEC) were positive for factor VIII-Rag, carbonic anhydrase IV, Ulex Europeus Agglutinin I, took up fluorescently labeled acetylated low density lipoprotein and expressed gamma glutamyl transpeptidase, demonstrating their brain endothelial cell characteristics. Upon treatment with TNFα, VCAM and ICAM but little ELAM was expressed on HBMEC, while VCAM, ICAM and ELAM were clearly evident on HUVEC. This selective expression of cell adhesion molecules was also demonstrated by in situ stimulation of brain tissues. In conclusion, microvascular endothelial cells from childrens brains display selective expression of cell adhesion molecules, which differ from macrovascular endothelial cells. This may have consequences for leukocyte trafficking into the central nervous system.

Original languageEnglish (US)
Pages (from-to)81-90
Number of pages10
JournalJournal of Neuroimmunology
Volume76
Issue number1-2
DOIs
StatePublished - Jun 1 1997
Externally publishedYes

Keywords

  • Cell adhesion molecules
  • Human cerebral microvessel endothelium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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