Selective export of MHC class I molecules from the ER after their dissociation from TAP

E. T. Spiliotis, M. Osorio, M. C. Zúñiga, M. Edidin

Research output: Contribution to journalArticle

Abstract

It has been assumed that upon dissociation from TAP, MHC class I molecules exit the ER by nonselective bulk flow. We now show that exit must occur by association with cargo receptors. Inconsistent with exit by bulk flow, loading of MHC class I molecules with high-affinity peptides triggers dissociation from TAP but has no effect on rates of ER-to-Golgi transport. Moreover, pepfide-loaded MHC class I molecules accumulate at ER exit sites from which TAP molecules are excluded. Consistent with receptor-mediated exit, ER-to-Golgi transport of MHC class I molecules is independent of their cytoplasmic tails, which themselves lack ER export motifs. In addition, we show that MHC class I molecules associate with the putative cargo receptor BAP31.

Original languageEnglish (US)
Pages (from-to)841-851
Number of pages11
JournalImmunity
Volume13
Issue number6
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Selective export of MHC class I molecules from the ER after their dissociation from TAP'. Together they form a unique fingerprint.

  • Cite this