TY - JOUR
T1 - Selective effects of interleukin (IL)-15 on antifungal activity and IL-8 release by polymorphonuclear leukocytes in response to hyphae of Aspergillus species
AU - Winn, Richard M.
AU - Gil-Lamaignere, Cristina
AU - Roilides, Emmanuel
AU - Simitsopoulou, Maria
AU - Lyman, Caron A.
AU - Maloukou, Avgi
AU - Walsh, Thomas J.
N1 - Funding Information:
Received 30 September 2002; accepted 21 March 2003; electronically published 29 July 2003. Presented in part: 12th Immunocompromised Host Society meeting, Bergen, Norway, 23–26 June 2002. Financial support: European Commission Training and Mobility of Researchers grant (TMR-EUROFUNG ERBFMXR-CT970145 to R.M.W. and C.G.-L.). a R.M.W. and C.G-L. contributed equally to this article. Reprints or correspondence: Dr. Thomas J. Walsh, Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Bldg. 10, Rm. 13N240, Bethesda, MD 20892 (walsht@mail.nih.gov).
PY - 2003/8/15
Y1 - 2003/8/15
N2 - The effects of interleukin (IL)-15 on human polymorphonuclear leukocyte (PMNL) activity against Aspergillus fumigatus and Aspergillus flavus were investigated. Pretreatment with IL-15 for 2 h increased PMNL oxidative burst, as measured by superoxide anion (O2-) release in response to A. fumigatus (P < .05) but not to A. flavus. However, after 22-h, but not 2-h, treatment with IL-15, there was significant enhancement in PMNL-mediated hyphal damage to A. fumigatus. Furthermore, 22-h exposure to IL-15 mediated an enhanced release of IL-8 from PMNLs challenged with hyphae of A. fumigatus and A. flavus (P < .05). In contrast, IL-15 treatment did not affect the release of tumor necrosis factor-α from PMNLs. The selective time- and species-dependent enhancement of O2- production and hyphal damage, as well as its induction of IL-8 release, suggest that IL-15 may play an important role in the immunomodulation of host response to invasive aspergillosis.
AB - The effects of interleukin (IL)-15 on human polymorphonuclear leukocyte (PMNL) activity against Aspergillus fumigatus and Aspergillus flavus were investigated. Pretreatment with IL-15 for 2 h increased PMNL oxidative burst, as measured by superoxide anion (O2-) release in response to A. fumigatus (P < .05) but not to A. flavus. However, after 22-h, but not 2-h, treatment with IL-15, there was significant enhancement in PMNL-mediated hyphal damage to A. fumigatus. Furthermore, 22-h exposure to IL-15 mediated an enhanced release of IL-8 from PMNLs challenged with hyphae of A. fumigatus and A. flavus (P < .05). In contrast, IL-15 treatment did not affect the release of tumor necrosis factor-α from PMNLs. The selective time- and species-dependent enhancement of O2- production and hyphal damage, as well as its induction of IL-8 release, suggest that IL-15 may play an important role in the immunomodulation of host response to invasive aspergillosis.
UR - http://www.scopus.com/inward/record.url?scp=0041439962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041439962&partnerID=8YFLogxK
U2 - 10.1086/377099
DO - 10.1086/377099
M3 - Article
C2 - 12898448
AN - SCOPUS:0041439962
SN - 0022-1899
VL - 188
SP - 585
EP - 590
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -