Abstract
A method termed selective differential fingerprinting (SDF) has been developed that enables one to investigate the level of expression for a family of genes between two samples. SDF produces a fingerprint (on a sequencing gel) on reverse transcription polymerase chain reaction (RT-PCR) of a sample with degenerate primers designed from conserved regions of a family of genes. By comparing fingerprints obtained after SDF with primers representing the transforming growth factor-beta (TGFβ) family of growth factors between a low-grade and a high-grade tumor from the same patient, a TGFβ family member known as osteogenic protein 1 (OP-1) or bone morphogenic protein 7 (BMP-7) was found to be greatly overexpressed in the high-grade tumor compared to the low-grade one. SDF also has the potential to identify novel genes. SDF offers a general way to identify differentially expressed genes for a family between two given samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 77-81 |
| Number of pages | 5 |
| Journal | Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
Keywords
- Differential display PCR
- Expression
- Gene family
- Nested PCR
- TGF-β
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology