Selective differential fingerprinting: A method for identifying differentially expressed genes in a family between two samples

Kristy L. Weber, Mark E. Bolander, Gobinda Sarkar

Research output: Contribution to journalArticlepeer-review

Abstract

A method termed selective differential fingerprinting (SDF) has been developed that enables one to investigate the level of expression for a family of genes between two samples. SDF produces a fingerprint (on a sequencing gel) on reverse transcription polymerase chain reaction (RT-PCR) of a sample with degenerate primers designed from conserved regions of a family of genes. By comparing fingerprints obtained after SDF with primers representing the transforming growth factor-beta (TGFβ) family of growth factors between a low-grade and a high-grade tumor from the same patient, a TGFβ family member known as osteogenic protein 1 (OP-1) or bone morphogenic protein 7 (BMP-7) was found to be greatly overexpressed in the high-grade tumor compared to the low-grade one. SDF also has the potential to identify novel genes. SDF offers a general way to identify differentially expressed genes for a family between two given samples.

Original languageEnglish (US)
Pages (from-to)77-81
Number of pages5
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Differential display PCR
  • Expression
  • Gene family
  • Nested PCR
  • TGF-β

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Selective differential fingerprinting: A method for identifying differentially expressed genes in a family between two samples'. Together they form a unique fingerprint.

Cite this