Selective contractile dysfunction of left, not right, ventricular myocardium in the SHHF rat

Paul M L Janssen; Linda B. Stull; Michelle K. Leppo; Ruth A. Altschuld; Eduardo Marban

Selective contractile dysfunction of left, not right, ventricular myocardium in the SHHF rat

Paul M L Janssen, Linda B. Stull, Michelle K. Leppo, Ruth A. Altschuld, Eduardo Marban

School of Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The progression of hypertension to cardiac failure involves systemic changes that may ultimately affect contractility throughout the heart. Spontaneous hypertensive heart failure (SHHF) rats have depressed left ventricular (LV) function, but right ventricular (RV) dysfunction is less well characterized. Ultrathin (87 ± 5 μm) trabeculae were isolated from end-stage failing SHHF rats and from age-matched controls. Under near-physiological conditions (1 mM Ca²⁺, 37°C, 4 Hz), developed force (in mN/mm²) was not significantly different in SHHF LV and RV trabeculae and those of controls. SHHF LV preparations displayed a negative force-frequency behavior (40 ± 7 vs. 23 ± 4 mN/mm², 2 vs. 7 Hz); this relationship was positive in SHHF RV preparations (27 ± 5 vs. 40 ± 6 mN/mm²) and controls (32 ± 6 vs. 44 ± 9 mN/mm²). The response to isoproterenol (10^-6 M, 4 Hz) was depressed in SHHF LV preparations. The inotropic response to hypothermia was lost in SHHF LV trabeculae but preserved in SHHF RV trabeculae. Intracellular calcium measurements revealed impaired calcium handling at higher frequencies in LV preparations. We conclude that in end-stage failing SHHF rats, RV function is only marginally affected, whereas a severe contractile dysfunction of LV myocardium is present.

Original language	English (US)
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	284
Issue number	3 53-3
State	Published - Mar 1 2003

Keywords

Contractile function
Excitation-contraction coupling
Heart failure
Hypertension
Inotropic agents

ASJC Scopus subject areas

Physiology

Cite this

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title = "Selective contractile dysfunction of left, not right, ventricular myocardium in the SHHF rat",

abstract = "The progression of hypertension to cardiac failure involves systemic changes that may ultimately affect contractility throughout the heart. Spontaneous hypertensive heart failure (SHHF) rats have depressed left ventricular (LV) function, but right ventricular (RV) dysfunction is less well characterized. Ultrathin (87 ± 5 μm) trabeculae were isolated from end-stage failing SHHF rats and from age-matched controls. Under near-physiological conditions (1 mM Ca2+, 37°C, 4 Hz), developed force (in mN/mm2) was not significantly different in SHHF LV and RV trabeculae and those of controls. SHHF LV preparations displayed a negative force-frequency behavior (40 ± 7 vs. 23 ± 4 mN/mm2, 2 vs. 7 Hz); this relationship was positive in SHHF RV preparations (27 ± 5 vs. 40 ± 6 mN/mm2) and controls (32 ± 6 vs. 44 ± 9 mN/mm2). The response to isoproterenol (10-6 M, 4 Hz) was depressed in SHHF LV preparations. The inotropic response to hypothermia was lost in SHHF LV trabeculae but preserved in SHHF RV trabeculae. Intracellular calcium measurements revealed impaired calcium handling at higher frequencies in LV preparations. We conclude that in end-stage failing SHHF rats, RV function is only marginally affected, whereas a severe contractile dysfunction of LV myocardium is present.",

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AU - Stull, Linda B.

AU - Leppo, Michelle K.

AU - Altschuld, Ruth A.

AU - Marban, Eduardo

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N2 - The progression of hypertension to cardiac failure involves systemic changes that may ultimately affect contractility throughout the heart. Spontaneous hypertensive heart failure (SHHF) rats have depressed left ventricular (LV) function, but right ventricular (RV) dysfunction is less well characterized. Ultrathin (87 ± 5 μm) trabeculae were isolated from end-stage failing SHHF rats and from age-matched controls. Under near-physiological conditions (1 mM Ca2+, 37°C, 4 Hz), developed force (in mN/mm2) was not significantly different in SHHF LV and RV trabeculae and those of controls. SHHF LV preparations displayed a negative force-frequency behavior (40 ± 7 vs. 23 ± 4 mN/mm2, 2 vs. 7 Hz); this relationship was positive in SHHF RV preparations (27 ± 5 vs. 40 ± 6 mN/mm2) and controls (32 ± 6 vs. 44 ± 9 mN/mm2). The response to isoproterenol (10-6 M, 4 Hz) was depressed in SHHF LV preparations. The inotropic response to hypothermia was lost in SHHF LV trabeculae but preserved in SHHF RV trabeculae. Intracellular calcium measurements revealed impaired calcium handling at higher frequencies in LV preparations. We conclude that in end-stage failing SHHF rats, RV function is only marginally affected, whereas a severe contractile dysfunction of LV myocardium is present.

AB - The progression of hypertension to cardiac failure involves systemic changes that may ultimately affect contractility throughout the heart. Spontaneous hypertensive heart failure (SHHF) rats have depressed left ventricular (LV) function, but right ventricular (RV) dysfunction is less well characterized. Ultrathin (87 ± 5 μm) trabeculae were isolated from end-stage failing SHHF rats and from age-matched controls. Under near-physiological conditions (1 mM Ca2+, 37°C, 4 Hz), developed force (in mN/mm2) was not significantly different in SHHF LV and RV trabeculae and those of controls. SHHF LV preparations displayed a negative force-frequency behavior (40 ± 7 vs. 23 ± 4 mN/mm2, 2 vs. 7 Hz); this relationship was positive in SHHF RV preparations (27 ± 5 vs. 40 ± 6 mN/mm2) and controls (32 ± 6 vs. 44 ± 9 mN/mm2). The response to isoproterenol (10-6 M, 4 Hz) was depressed in SHHF LV preparations. The inotropic response to hypothermia was lost in SHHF LV trabeculae but preserved in SHHF RV trabeculae. Intracellular calcium measurements revealed impaired calcium handling at higher frequencies in LV preparations. We conclude that in end-stage failing SHHF rats, RV function is only marginally affected, whereas a severe contractile dysfunction of LV myocardium is present.

KW - Contractile function

KW - Excitation-contraction coupling

KW - Heart failure

KW - Hypertension

KW - Inotropic agents

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ER -

Selective contractile dysfunction of left, not right, ventricular myocardium in the SHHF rat

Abstract

Keywords

ASJC Scopus subject areas

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