Selective association between MHC class I-restricted T cell receptors, CD8, and activated tyrosine kinases on thymocytes undergoing positive selection

A. C. Carrera, S. B. Wee, T. M. Roberts, D. M. Pardoll

Research output: Contribution to journalArticlepeer-review

Abstract

Developing T cells undergo distinct selection processes that determine the TCR repertoire. Positive selection involves the differentiation of immature thymocytes capable of recognizing antigens complexed with self-MHC molecules to mature T cells. Besides the central role of TCR engagement by MHC in triggering selection; the interaction of CD8 and CD4 with MHC class I and class II, respectively; is thought to be important in regulating the selection process. To study potential mechanisms involved in positive selection of CD8+ cells, we have analyzed mice expressing a unique transgenic TCR. The transgenic receptor recognizes the HY male Ag in the context of the MHC class I molecule, H2-Db. We describe that CD8 and the TCR are selectively associated in thymocytes of mice expressing the restricting MHC, but not in thymocytes of mice expressing a nonrestricting MHC, pp56(lck) and pp59(fyn), the tyrosine kinases associated with CD8 and TCR, respectively, were found to be present in this complex in an activated form. No comparable TCR-CD4 complex formation was found in thymuses undergoing positive selection to CD8+ cells. The formation of a multimolecular complex between CD8 and TCR, in which pp56(lck) and pp59(fyn) are activated, may initiate specific signaling programs involved in the maturation of CD8+ cells.

Original languageEnglish (US)
Pages (from-to)3142-3149
Number of pages8
JournalJournal of Immunology
Volume149
Issue number10
StatePublished - 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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