TY - JOUR
T1 - Selective and nonselective packaging of cellular RNAs in retrovirus particles
AU - Rulli, Samuel J.
AU - Hibbert, Catherine S.
AU - Mirro, Jane
AU - Pederson, Thoru
AU - Biswal, Shyam
AU - Rein, Alan
PY - 2007/6
Y1 - 2007/6
N2 - Assembly of retrovirus particles normally entails the selective encapsidation of viral genomic RNA. However, in the absence of packageable viral RNA, assembly is still efficient, and the released virus-like particles (termed "ψ-" particles) still contain roughly normal amounts of RNA. We have proposed that cellular mRNAs replace the genome in ψ- particles. We have now analyzed the mRNA content of ψ- and ψ+ murine leukemia virus (MLV) particles using both microarray analysis and real-time reverse transcription-PCR. The majority of mRNA species present in the virus-producing cells were also detected in ψ- particles. Remarkably, nearly all of them were packaged nonselectively; that is, their representation in the particles was simply proportional to their representation in the cells. However, a small number of low-abundance mRNAs were greatly enriched in the particles. In fact, one mRNA species was enriched to the same degree as ψ+ genomic RNA. Similar results were obtained with particles formed from the human immunodeficiency virus type 1 (HIV-1) Gag protein, and the same mRNAs were enriched in MLV and HIV-1 particles. The levels of individual cellular mRNAs were ∼5-to 10-fold higher in ψ- than in ψ+ MLV particles, in agreement with the idea that they are replacing viral RNA in the former. In contrast, signal recognition particle RNA was present at the same level in ψ- and ψ+ particles; a minor fraction of this RNA was weakly associated with genomic RNA in ψ+ MLV particles.
AB - Assembly of retrovirus particles normally entails the selective encapsidation of viral genomic RNA. However, in the absence of packageable viral RNA, assembly is still efficient, and the released virus-like particles (termed "ψ-" particles) still contain roughly normal amounts of RNA. We have proposed that cellular mRNAs replace the genome in ψ- particles. We have now analyzed the mRNA content of ψ- and ψ+ murine leukemia virus (MLV) particles using both microarray analysis and real-time reverse transcription-PCR. The majority of mRNA species present in the virus-producing cells were also detected in ψ- particles. Remarkably, nearly all of them were packaged nonselectively; that is, their representation in the particles was simply proportional to their representation in the cells. However, a small number of low-abundance mRNAs were greatly enriched in the particles. In fact, one mRNA species was enriched to the same degree as ψ+ genomic RNA. Similar results were obtained with particles formed from the human immunodeficiency virus type 1 (HIV-1) Gag protein, and the same mRNAs were enriched in MLV and HIV-1 particles. The levels of individual cellular mRNAs were ∼5-to 10-fold higher in ψ- than in ψ+ MLV particles, in agreement with the idea that they are replacing viral RNA in the former. In contrast, signal recognition particle RNA was present at the same level in ψ- and ψ+ particles; a minor fraction of this RNA was weakly associated with genomic RNA in ψ+ MLV particles.
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U2 - 10.1128/JVI.02833-06
DO - 10.1128/JVI.02833-06
M3 - Article
C2 - 17392359
AN - SCOPUS:34249939912
SN - 0022-538X
VL - 81
SP - 6623
EP - 6631
JO - Journal of virology
JF - Journal of virology
IS - 12
ER -