Selection of antifolate-resistant Plasmodium falciparum by sulfadoxine-pyrimethamine treatment and infectivity to Anopheles mosquitoes

Fabián Méndez, Sócrates Herrera, Bermans Murrain, Andrés Gutiérrez, Luz A. Moreno, María Manzano, Alvaro Munoz, Christopher V. Plowe

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Abstract

Resistance-conferring mutations in dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) in Plasmodium falciparum are selected by treatment with sulfadoxine pyrimethamine (SP). We assessed the association between these mutations and transmission capacity of parasites to Anopheles mosquitoes on the Pacific coast of Colombia. Patients with uncomplicated P. falciparum malaria received SP treatment and were followed-up to compare the prevalence of DHFR and DHPS mutations before and after SP treatment. Membrane feeding assays were used to measure infectivity to mosquitoes of post-treatment gametocytes with and without these mutations. Per-protocol treatment efficacy was 95.0% (132 of 139). Gametocytes carrying resistance-conferring mutations were selected after SP treatment and were infective to mosquitoes. Seven days after treatment, infections with two DHFF1 mutations had a 10-fold higher probability of infecting mosquitoes than infections with no DHFR mutations (odds ratio = 10.23, P <0.05). Low-level drug resistance mutations have the potential to enhance transmission of P. falciparum and spread resistant parasites.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number3
Publication statusPublished - Sep 2007

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ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Méndez, F., Herrera, S., Murrain, B., Gutiérrez, A., Moreno, L. A., Manzano, M., ... Plowe, C. V. (2007). Selection of antifolate-resistant Plasmodium falciparum by sulfadoxine-pyrimethamine treatment and infectivity to Anopheles mosquitoes. American Journal of Tropical Medicine and Hygiene, 77(3), 438-443.