Seizure-induced memory impairment is reduced by choline supplementation before or after status epilepticus

Gregory L. Holmes, Yili Yang, Zhao Liu, Jennifer M. Cermak, Matthew R. Sarkisian, Carl Stafstrom, John C. Neill, Jan K. Blusztajn

Research output: Contribution to journalArticle

Abstract

Prenatal choline supplementation can protect rats against cognitive deficits induced by status epilepticus induced by the cholinergic agent pilocarpine [J. Neurosci. 20 (2000) 1]. In the present day, we have extended this novel finding by investigating the effects of pre- and postnatal choline supplementation in memory deficits associated with status epilepticus induced with kainic acid (KA). In the first experiment pregnant rats received a normal, choline-supplemented, or choline deficient diet starting on the 11th day of gestation and continuing until postnatal (P) 7. At P42, rats were given a convulsant dosage of KA. Two weeks following the KA-induced status epilepticus rats underwent testing of visual-spatial memory using the Morris water maze test. Rats receiving supplemental choline performed better in the water maze than the deficient and control groups. Moreover, the activity of hippocampal choline acetyltransferase was 18% lower in the choline deficient animals as compared with the other two groups. In the second experiment we administered KA to P35 rats that had been given a normal diet. Following the status epilepticus the rats were given a choline-supplemented or control diet for 4 weeks and then tested in the water maze. Rats receiving choline supplementation performed far better than rats receiving a regular diet. This study demonstrates that choline supplementation prior to or following KA-induced status epilepticus can protect rats from memory deficits induced by status epilepticus.

Original languageEnglish (US)
Pages (from-to)3-13
Number of pages11
JournalEpilepsy Research
Volume48
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Status Epilepticus
Choline
Seizures
Kainic Acid
Diet
Memory Disorders
Water
Convulsants
Pilocarpine
Choline O-Acetyltransferase
Cholinergic Agents
Pregnancy
Control Groups

Keywords

  • Behavior
  • Epilepsy
  • Hippocampus
  • Kainic acid
  • Learning
  • Pilocarpine
  • Seizures
  • Water maze

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology

Cite this

Seizure-induced memory impairment is reduced by choline supplementation before or after status epilepticus. / Holmes, Gregory L.; Yang, Yili; Liu, Zhao; Cermak, Jennifer M.; Sarkisian, Matthew R.; Stafstrom, Carl; Neill, John C.; Blusztajn, Jan K.

In: Epilepsy Research, Vol. 48, No. 1-2, 2002, p. 3-13.

Research output: Contribution to journalArticle

Holmes, GL, Yang, Y, Liu, Z, Cermak, JM, Sarkisian, MR, Stafstrom, C, Neill, JC & Blusztajn, JK 2002, 'Seizure-induced memory impairment is reduced by choline supplementation before or after status epilepticus', Epilepsy Research, vol. 48, no. 1-2, pp. 3-13. https://doi.org/10.1016/S0920-1211(01)00321-7
Holmes, Gregory L. ; Yang, Yili ; Liu, Zhao ; Cermak, Jennifer M. ; Sarkisian, Matthew R. ; Stafstrom, Carl ; Neill, John C. ; Blusztajn, Jan K. / Seizure-induced memory impairment is reduced by choline supplementation before or after status epilepticus. In: Epilepsy Research. 2002 ; Vol. 48, No. 1-2. pp. 3-13.
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