Securin is required for chromosomal stability in human cells

Prasad V. Jallepalli, Irene C. Waizenegger, Fred Bunz, Sabine Langer, Michael R. Speicher, Jan Michael Peters, Kenneth W. Kinzler, Bert Vogelstein, Christoph Lengauer

Research output: Contribution to journalArticlepeer-review

Abstract

Abnormalities of chromosome number are the most common genetic aberrations in cancer. The mechanisms regulating the fidelity of mitotic chromosome transmission in mammalian cells are therefore of great interest. Here we show that human cells without an hSecurin gene lose chromosomes at a high frequency. This loss was linked to abnormal anaphases during which cells underwent repetitive unsuccessful attempts to segregate their chromosomes. The abnormal mitoses were associated with biochemical defects in the activation of separin, the sister-separating protease, rendering it unable to cleave the cohesin subunit Scc1 efficiently. These results illuminate the function of mammalian securin and show that it is essential for the maintenance of euploidy.

Original languageEnglish (US)
Pages (from-to)445-457
Number of pages13
JournalCell
Volume105
Issue number4
DOIs
StatePublished - May 18 2001

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Securin is required for chromosomal stability in human cells'. Together they form a unique fingerprint.

Cite this