Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach

Shmuel Batzri; John W. Harmon; Michael D. Walker; William F. Thompson; Raymond Toles

doi:10.1016/0167-4889(82)90015-5

Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach

Shmuel Batzri, John W. Harmon, Michael D. Walker, William F. Thompson, Raymond Toles

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37°C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cell preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (K_i=5·10^-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H₂-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.

Original language	English (US)
Pages (from-to)	217-221
Number of pages	5
Journal	BBA - Molecular Cell Research
Volume	720
Issue number	2
DOIs	https://doi.org/10.1016/0167-4889(82)90015-5
State	Published - Apr 29 1982
Externally published	Yes

Keywords

(Mucosal cell)
H receptor
Histamine
Intrinsic factor secretion
Isobutyl methylxanthine

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/0167-4889(82)90015-5

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title = "Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach",

abstract = "In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37°C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cell preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (Ki=5·10-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H2-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.",

keywords = "(Mucosal cell), H receptor, Histamine, Intrinsic factor secretion, Isobutyl methylxanthine",

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AU - Batzri, Shmuel

AU - Harmon, John W.

AU - Walker, Michael D.

AU - Thompson, William F.

AU - Toles, Raymond

PY - 1982/4/29

Y1 - 1982/4/29

N2 - In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37°C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cell preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (Ki=5·10-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H2-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.

AB - In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37°C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cell preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (Ki=5·10-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H2-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.

KW - (Mucosal cell)

KW - H receptor

KW - Histamine

KW - Intrinsic factor secretion

KW - Isobutyl methylxanthine

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Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach

Abstract

Keywords

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