Secretion of intrinsic factor from dispersed mucosal cells isolated from guinea pig and rabbit stomach

Shmuel Batzri, John W. Harmon, Michael D. Walker, William F. Thompson, Raymond Toles

Research output: Contribution to journalArticlepeer-review

Abstract

In dispersed mucosal cells prepared from rabbit and guinea pig stomach, the secretion of intrinsic factor was constant (0.3-0.4%/min) for at least 30 min incubation at 37°C. Histamine or isobutyl methylxanthine increased cyclic AMP and intrinsic factor secretion in both cell preparations. Isobutyl methylxanthine potentiated and cimetidine competitively inhibited (Ki=5·10-7 M) both effects of histamine. Dibutyryl cyclic AMP (1.0 mM), also caused a 3-fold increase in intrinsic factor secretion. These results suggest that in rabbit and guinea pig histamine interacts with H2-receptors to increase cyclic AMP which mediates the rise in the rate of intrinsic factor secretion.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalBBA - Molecular Cell Research
Volume720
Issue number2
DOIs
StatePublished - Apr 29 1982
Externally publishedYes

Keywords

  • (Mucosal cell)
  • H receptor
  • Histamine
  • Intrinsic factor secretion
  • Isobutyl methylxanthine

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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