Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-β Induced Bone Formation Differently

Haijun Tian, Xiaoda Bi, Chen Shuang Li, Ke Wei Zhao, Elsa J. Brochmann, Scott R. Montgomery, Bayan Aghdasi, Deyu Chen, Michael D. Daubs, Jeffrey C. Wang, Samuel S. Murray

Research output: Contribution to journalArticle

Abstract

Transforming growth factor-β (TGF-β) and bone morphogenetic proteins (BMPs) have opposing but complementary functions in directing bone growth, repair, and turnover. Both are found in the bone matrix. Proteins that bind to and affect the activity of these growth factors will determine the relative abundance of the growth factors and, therefore, regulate bone formation. Secreted phosphoprotein 24 kD (Spp24) is a bone matrix protein that has been demonstrated to bind to and affect the activity of BMPs. The arginine-rich carboxy terminus of Spp24 is proteolytically processed to produce three other predictable truncation products (Spp18.1, Spp16.0, and Spp14.5). In this work, we report that kinetic data obtained by surface plasmon resonance demonstrate that Spp24 and the three C-terminal truncation products all bind to TGF-β1 and TGF-β2 with a similar but somewhat less affinity than they bind BMP-2; that, as in the case of BMP-2, the full-length (FL) form of Spp24 binds TGF-β with greater affinity than do the truncation products; that FL-Spp24 inhibits TGF-β2 induced bone formation in vivo, but Spp14.5 does not; and that co-administration of FL-Spp24 or Spp14.5 with TGF-β2 in vivo is associated with a reduction in the amount of cartilage, relative to new bone, present at the site of injection. This finding is consistent with the observation that low-dose TGF-β administration in vivo is associated with greater bone formation than high-dose TGF-β administration, and suggests that one function of Spp24 and its truncation products is to down-regulate local TGF-β activity or availability during bone growth and development. The similarities and differences of the interactions between Spp24 proteins and TGF-β compared to the interaction of the Spp24 proteins and BMPs have significant implications with respect to the regulation of bone metabolism and with respect to engineering therapeutic proteins for skeletal disorders.

Original languageEnglish (US)
Article numbere72645
JournalPloS one
Volume8
Issue number8
DOIs
StatePublished - Aug 26 2013
Externally publishedYes

Fingerprint

transforming growth factors
phosphoproteins
Transforming Growth Factors
bone formation
Osteogenesis
Bone
bone morphogenetic proteins
Bone Morphogenetic Proteins
bones
Bone Development
Bone Morphogenetic Protein 2
Bone Matrix
growth factors
Intercellular Signaling Peptides and Proteins
Proteins
proteins
secreted phosphoprotein 24
Protein Engineering
Bone and Bones
biopharmaceuticals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Tian, H., Bi, X., Li, C. S., Zhao, K. W., Brochmann, E. J., Montgomery, S. R., ... Murray, S. S. (2013). Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-β Induced Bone Formation Differently. PloS one, 8(8), [e72645]. https://doi.org/10.1371/journal.pone.0072645

Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-β Induced Bone Formation Differently. / Tian, Haijun; Bi, Xiaoda; Li, Chen Shuang; Zhao, Ke Wei; Brochmann, Elsa J.; Montgomery, Scott R.; Aghdasi, Bayan; Chen, Deyu; Daubs, Michael D.; Wang, Jeffrey C.; Murray, Samuel S.

In: PloS one, Vol. 8, No. 8, e72645, 26.08.2013.

Research output: Contribution to journalArticle

Tian, H, Bi, X, Li, CS, Zhao, KW, Brochmann, EJ, Montgomery, SR, Aghdasi, B, Chen, D, Daubs, MD, Wang, JC & Murray, SS 2013, 'Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-β Induced Bone Formation Differently', PloS one, vol. 8, no. 8, e72645. https://doi.org/10.1371/journal.pone.0072645
Tian, Haijun ; Bi, Xiaoda ; Li, Chen Shuang ; Zhao, Ke Wei ; Brochmann, Elsa J. ; Montgomery, Scott R. ; Aghdasi, Bayan ; Chen, Deyu ; Daubs, Michael D. ; Wang, Jeffrey C. ; Murray, Samuel S. / Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-β Induced Bone Formation Differently. In: PloS one. 2013 ; Vol. 8, No. 8.
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abstract = "Transforming growth factor-β (TGF-β) and bone morphogenetic proteins (BMPs) have opposing but complementary functions in directing bone growth, repair, and turnover. Both are found in the bone matrix. Proteins that bind to and affect the activity of these growth factors will determine the relative abundance of the growth factors and, therefore, regulate bone formation. Secreted phosphoprotein 24 kD (Spp24) is a bone matrix protein that has been demonstrated to bind to and affect the activity of BMPs. The arginine-rich carboxy terminus of Spp24 is proteolytically processed to produce three other predictable truncation products (Spp18.1, Spp16.0, and Spp14.5). In this work, we report that kinetic data obtained by surface plasmon resonance demonstrate that Spp24 and the three C-terminal truncation products all bind to TGF-β1 and TGF-β2 with a similar but somewhat less affinity than they bind BMP-2; that, as in the case of BMP-2, the full-length (FL) form of Spp24 binds TGF-β with greater affinity than do the truncation products; that FL-Spp24 inhibits TGF-β2 induced bone formation in vivo, but Spp14.5 does not; and that co-administration of FL-Spp24 or Spp14.5 with TGF-β2 in vivo is associated with a reduction in the amount of cartilage, relative to new bone, present at the site of injection. This finding is consistent with the observation that low-dose TGF-β administration in vivo is associated with greater bone formation than high-dose TGF-β administration, and suggests that one function of Spp24 and its truncation products is to down-regulate local TGF-β activity or availability during bone growth and development. The similarities and differences of the interactions between Spp24 proteins and TGF-β compared to the interaction of the Spp24 proteins and BMPs have significant implications with respect to the regulation of bone metabolism and with respect to engineering therapeutic proteins for skeletal disorders.",
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AU - Zhao, Ke Wei

AU - Brochmann, Elsa J.

AU - Montgomery, Scott R.

AU - Aghdasi, Bayan

AU - Chen, Deyu

AU - Daubs, Michael D.

AU - Wang, Jeffrey C.

AU - Murray, Samuel S.

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