TY - JOUR
T1 - Secreted and cell surface genes expressed in benign and malignant colorectal tumors
AU - Buckhaults, Phillip
AU - Rago, Carlo
AU - Vogelstein, Bert
AU - St. Croix, Brad
AU - Romans, Katharine E.
AU - Saha, Saurabh
AU - Zhang, Lin
AU - Kinzler, Kenneth W.
PY - 2001/10/1
Y1 - 2001/10/1
N2 - Serial analysis of gene expression was used to identify transcripts encoding secreted or cell surface proteins that were expressed in benign and malignant tumors of the colorectum. A total of 290,394 tags were analyzed from normal, adenomatous, and cancerous colonic epithelium. Of the 21,343 different transcripts observed, 957 were found to be differentially expressed between normal tissue and adenoma or between normal tissue and cancer. Forty-nine transcripts were elevated ≥20-fold in adenomas, 40 transcripts were elevated ≥20-fold in cancers, and 9 transcripts were elevated ≥20-fold in both. Products of six of these nine transcripts (TGFB1, LYS, RDP, MIC-1, REGA, and DEHL) were predicted to be secreted or to reside on the cell surface, and these were analyzed in more detail. The abnormal expression levels predicted by serial analysis of gene expression were confirmed by quantitative PCR analyses of each of these six genes. Moreover, the cell types responsible for the elevated expression were identified by in situ hybridization and by PCR analyses of epithelial cells immunoaffinity purified from primary tumors. This study extends knowledge of the differences in gene expression that underlie various stages of neoplasia and suggests specific diagnostic approaches that may be useful for the early detection of colorectal neoplasia.
AB - Serial analysis of gene expression was used to identify transcripts encoding secreted or cell surface proteins that were expressed in benign and malignant tumors of the colorectum. A total of 290,394 tags were analyzed from normal, adenomatous, and cancerous colonic epithelium. Of the 21,343 different transcripts observed, 957 were found to be differentially expressed between normal tissue and adenoma or between normal tissue and cancer. Forty-nine transcripts were elevated ≥20-fold in adenomas, 40 transcripts were elevated ≥20-fold in cancers, and 9 transcripts were elevated ≥20-fold in both. Products of six of these nine transcripts (TGFB1, LYS, RDP, MIC-1, REGA, and DEHL) were predicted to be secreted or to reside on the cell surface, and these were analyzed in more detail. The abnormal expression levels predicted by serial analysis of gene expression were confirmed by quantitative PCR analyses of each of these six genes. Moreover, the cell types responsible for the elevated expression were identified by in situ hybridization and by PCR analyses of epithelial cells immunoaffinity purified from primary tumors. This study extends knowledge of the differences in gene expression that underlie various stages of neoplasia and suggests specific diagnostic approaches that may be useful for the early detection of colorectal neoplasia.
UR - http://www.scopus.com/inward/record.url?scp=0035477337&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035477337&partnerID=8YFLogxK
M3 - Article
C2 - 11585723
AN - SCOPUS:0035477337
VL - 61
SP - 6996
EP - 7001
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 19
ER -