Secretagogue-induced redistributions of Na,K-ATPase in rat lacrimal acini

Samuel Chi-Hung Yiu, R. W. Lambert, P. J. Tortoriello, A. K. Mircheff

Research output: Contribution to journalArticle

Abstract

Lacrimal acinar cells secrete macromolecular products in an approximately isotonic, sodium chloride (NaCl)-rich fluid. The mechanisms of macromolecular product secretion depend in part on a recycling traffic of membrane constituents between the Golgi complex and the apical plasma membrane. In contrast, the acinar cell's mechanisms for secreting Na+ and Cl- depend largely on the fluxes of these ions through transporters expressed in the apical and basal-lateral membranes. In addition to accelerating the recycling of secretory vesicle membrane constituents, the cholinergic agonist carbachol also triggers a net redistribution of sodium potassium adenosine triphosphatase (Na,K-ATPase) ion pumps between Golgi-associated pools and the basal-lateral plasma membranes (Yiu SC, et al: J Membrane Biol 102:185, 1988). In the present study, acinar preparations from rat lacrimal glands were stimulated with either carbachol, epinephrine, or isoproterenol. All three agonists stimulated release of the secretory protein lactoperoxidase, but only carbachol significantly accelerated Na+ unidirectional influx. Subcellular fractionation analyses of resting and stimulated preparations indicated that carbachol caused a significant translocation of Na,K-ATPase activity from a Golgi-associated compartment to the basal-lateral plasma membranes. Neither adrenergic agonist significantly increased the basal-lateral membrane Na,K-ATPase activity, but each triggered a distinct pattern of redistributions of Na,K-ATPase and the Golgi membrane marker, galactosyltransferase. The carbachol-induced augmentation of basal-lateral membrane Na,K-ATPase activity represents a mechanism by which the cell might compensate for increased Na+ influx. However, it remains to be determined whether the signal for Na,K-ATPase recruitment is an elevation of cytosolic Na+ activity or whether the redistribution is mediated more directly by the intracellular messenger systems activated after cholinergic stimulation.

Original languageEnglish (US)
Pages (from-to)2976-2984
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume32
Issue number11
StatePublished - 1991
Externally publishedYes

Fingerprint

Tears
Carbachol
Membranes
Acinar Cells
Cell Membrane
Ion Pumps
Lactoperoxidase
Galactosyltransferases
Cholinergic Agonists
Sodium-Potassium-Exchanging ATPase
Lacrimal Apparatus
Adrenergic Agonists
Secretory Vesicles
Recycling
Golgi Apparatus
sodium-translocating ATPase
Isoproterenol
Sodium Chloride
Cholinergic Agents
Epinephrine

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Secretagogue-induced redistributions of Na,K-ATPase in rat lacrimal acini. / Yiu, Samuel Chi-Hung; Lambert, R. W.; Tortoriello, P. J.; Mircheff, A. K.

In: Investigative Ophthalmology and Visual Science, Vol. 32, No. 11, 1991, p. 2976-2984.

Research output: Contribution to journalArticle

Yiu, SC-H, Lambert, RW, Tortoriello, PJ & Mircheff, AK 1991, 'Secretagogue-induced redistributions of Na,K-ATPase in rat lacrimal acini', Investigative Ophthalmology and Visual Science, vol. 32, no. 11, pp. 2976-2984.
Yiu, Samuel Chi-Hung ; Lambert, R. W. ; Tortoriello, P. J. ; Mircheff, A. K. / Secretagogue-induced redistributions of Na,K-ATPase in rat lacrimal acini. In: Investigative Ophthalmology and Visual Science. 1991 ; Vol. 32, No. 11. pp. 2976-2984.
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