TY - JOUR
T1 - Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs
AU - Mostaco-Guidolin, Leila B.
AU - Loube, Jeffrey
AU - Barlow, Aaron
AU - Osei, Emmanuel T.
AU - Vasilescu, Dragoș șM
AU - Hsieh, Aileen
AU - Fouadi, May
AU - Young, Christine
AU - Scott, Alan L.
AU - Mitzner, Wayne
AU - Hackett, Tillie L.
N1 - Funding Information:
This work is funded by an unrestricted research grant from the Canadian Institutes of Health Research grant (MOP 1305040) and from the Heart, Blood and Lung Institute of the National Institutes of Health R01-HL140623. Dr. Osei holds Michael Smith Foundation for Health Research (MSFHR) and a MITACS Accelerate postdoctoral fellowships in the Providence Airway Center, UBC. Dr. Vasilescu is supported by the Parker B. Francis Foundation. Dr. Hackett is supported by CIHR, MSFHR, and Parker B. Francis Foundation New Investigator awards.
Funding Information:
This work is funded by an unrestricted research grant from the Canadian Institutes of Health Research grant (MOP 1305040) and from the Heart, Blood and Lung Institute of the National Institutes of Health R01-HL140623. Dr. Osei holds Michael Smith Foundation for Health Research (MSFHR) and a MITACS Accelerate postdoctoral fellowships in the Providence Airway Center, UBC. Dr. Vasilescu is supported by the Parker B. Francis Foundation. Dr. Hackett is supported by CIHR, MSFHR, and Parker B. Francis Foundation New Investigator awards.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
PY - 2021/2
Y1 - 2021/2
N2 - The alveolar ducts are connected to peripheral septal fibers which extend from the visceral pleura into interlobular septa, and are anchored to axial fibers in the small airways. Together these axial and septal fibers constitute a fiber continuum that provides tension and integrity throughout the lung. Building on the observations that alveolar ducts associated with sub-pleural alveoli are orientated perpendicular to the visceral pleura, and in parallel to each other, the goal of the present study was to investigate the nature of the collagen fiber organization within sub-pleural alveolar ducts in healthy control and elastase-induced emphysema murine lungs. Employing three-dimensional second harmonic generation imaging, the structural arrangement of fibrilar collagen fibers could be visualized in cleared murine lungs. In healthy control lungs, fibrilar collagen fibers within alveolar mouths formed the coiled collagen structure within the alveolar duct. In the elastase-treated emphysema lungs, there was loss of fibrilar collagen fibers (p < 0.01) and disruption of collagens structural organization as measured by the fibrillar collagen length (p < 0.01) and entropy (p < 0.01). Compared to the alveolar ducts from healthy controls, there was a significant increase in the area of cells (nm2, p < 0.001), and area of cells with cytoplasmic granules (nm2, p < 0.001) compared to emphysematous lungs. These results are consistent with the idea that one of the major contributors to the progressive loss of alveolar surfaces and elastic recoil in the emphysematous lung is loss of the structural integrity of the collagen scaffold that maintains the spatial relationships important for cell survival and lung function.
AB - The alveolar ducts are connected to peripheral septal fibers which extend from the visceral pleura into interlobular septa, and are anchored to axial fibers in the small airways. Together these axial and septal fibers constitute a fiber continuum that provides tension and integrity throughout the lung. Building on the observations that alveolar ducts associated with sub-pleural alveoli are orientated perpendicular to the visceral pleura, and in parallel to each other, the goal of the present study was to investigate the nature of the collagen fiber organization within sub-pleural alveolar ducts in healthy control and elastase-induced emphysema murine lungs. Employing three-dimensional second harmonic generation imaging, the structural arrangement of fibrilar collagen fibers could be visualized in cleared murine lungs. In healthy control lungs, fibrilar collagen fibers within alveolar mouths formed the coiled collagen structure within the alveolar duct. In the elastase-treated emphysema lungs, there was loss of fibrilar collagen fibers (p < 0.01) and disruption of collagens structural organization as measured by the fibrillar collagen length (p < 0.01) and entropy (p < 0.01). Compared to the alveolar ducts from healthy controls, there was a significant increase in the area of cells (nm2, p < 0.001), and area of cells with cytoplasmic granules (nm2, p < 0.001) compared to emphysematous lungs. These results are consistent with the idea that one of the major contributors to the progressive loss of alveolar surfaces and elastic recoil in the emphysematous lung is loss of the structural integrity of the collagen scaffold that maintains the spatial relationships important for cell survival and lung function.
KW - Alveolar duct
KW - COPD
KW - Collagen
KW - Second harmonic imaging
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U2 - 10.1007/s00418-020-01959-6
DO - 10.1007/s00418-020-01959-6
M3 - Article
C2 - 33515079
AN - SCOPUS:85099929503
SN - 0948-6143
VL - 155
SP - 279
EP - 289
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 2
ER -