Second-hand cigarette smoke impairs bacterial phagocytosis in macrophages by modulating CFTR dependent lipid-rafts

Inzer Ni, Changhoon Ji, Neeraj Vij

Research output: Contribution to journalArticle

Abstract

Introduction: First/Second-hand cigarette-smoke (FHS/SHS) exposure weakens immune defenses inducing chronic obstructive pulmonary disease (COPD) but the underlying mechanisms are not fully understood. Hence, we evaluated if SHS induced changes in membrane/lipid-raft (m-/r)-CFTR (cystic fibrosis transmembrane conductance regulator) expression/activity is a potential mechanism for impaired bacterial phagocytosis in COPD. Methods: RAW264.7 murine macrophages were exposed to freshly prepared CS-extract (CSE) containing culture media and/or Pseudomonas-aeruginosa-PA01-GFP for phagocytosis (fluorescence-microscopy), bacterial survival (colony-forming-units-CFU), and immunoblotting assays. The CFTR-expression/activity and lipid-rafts were modulated by transient-transfection or inhibitors/inducers. Next, mice were exposed to acute/sub-chronic-SHS or room-air (5-days/3-weeks) and infected with PA01-GFP, followed by quantification of bacterial survival by CFU-assay. Results: We investigated the effect of CSE treatment on RAW264.7 cells infected by PA01-GFP and observed that CSE treatment significantly (p

Original languageEnglish (US)
Article numbere0121200
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 20 2015

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Pulmonary diseases
Tobacco Smoke Pollution
cigarettes
Macrophages
smoke
phagocytosis
Phagocytosis
Smoke
Tobacco Products
Chronic Obstructive Pulmonary Disease
Assays
macrophages
hands
Lipids
Cystic Fibrosis Transmembrane Conductance Regulator
respiratory tract diseases
Fluorescence microscopy
extracts
Membrane Lipids
lipids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Second-hand cigarette smoke impairs bacterial phagocytosis in macrophages by modulating CFTR dependent lipid-rafts. / Ni, Inzer; Ji, Changhoon; Vij, Neeraj.

In: PLoS One, Vol. 10, No. 3, e0121200, 20.03.2015.

Research output: Contribution to journalArticle

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