Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans

John Panepinto, Kazimierz Komperda, Susana Frases, Yoon Dong Park, Julianne T. Djordjevic, Arturo Casadevall, Peter R. Williamson

Research output: Contribution to journalArticle

Abstract

The cell wall of pathogenic fungi such as Cryptococcus neoformans, provides a formidable barrier to secrete virulence factors that produce host cell damage. To study secretion of virulence factors to the cell periphery, sec6 RNAi mutant strains of C. neoformans were tested for virulence factor expression. The studies reported here show that SEC6 RNAi mutant strains were defective in a number of virulence factors including laccase, urease as well as soluble polysaccharide and demonstrated attenuated virulence in mice. Further analysis by transmission electron microscopy detected the production of abundant extracellular exosomes in wild-type strains containing empty plasmid, but a complete absence in the iSEC6 strain. In addition, a green fluorescent protein-laccase fusion protein demonstrated aberrant localization within cytoplasmic vesicles in iSEC6 strains. In contrast, iSEC6 strains retained normal growth at 37°C, as well as substantially normal capsule formation, phospholipase activity and total secreted protein. These results provide the first molecular evidence for the existence of fungal exosomes and associate these vesicles with the virulence of C. neoformans.

Original languageEnglish (US)
Pages (from-to)1165-1176
Number of pages12
JournalMolecular Microbiology
Volume71
Issue number5
DOIs
StatePublished - Mar 2009
Externally publishedYes

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Exosomes
Laccase
Cryptococcus neoformans
Virulence Factors
RNA Interference
Virulence
Cytoplasmic Vesicles
Urease
Phospholipases
Green Fluorescent Proteins
Transmission Electron Microscopy
Cell Wall
Capsules
Polysaccharides
Proteins
Plasmids
Fungi
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Panepinto, J., Komperda, K., Frases, S., Park, Y. D., Djordjevic, J. T., Casadevall, A., & Williamson, P. R. (2009). Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. Molecular Microbiology, 71(5), 1165-1176. https://doi.org/10.1111/j.1365-2958.2008.06588.x

Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. / Panepinto, John; Komperda, Kazimierz; Frases, Susana; Park, Yoon Dong; Djordjevic, Julianne T.; Casadevall, Arturo; Williamson, Peter R.

In: Molecular Microbiology, Vol. 71, No. 5, 03.2009, p. 1165-1176.

Research output: Contribution to journalArticle

Panepinto, J, Komperda, K, Frases, S, Park, YD, Djordjevic, JT, Casadevall, A & Williamson, PR 2009, 'Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans', Molecular Microbiology, vol. 71, no. 5, pp. 1165-1176. https://doi.org/10.1111/j.1365-2958.2008.06588.x
Panepinto, John ; Komperda, Kazimierz ; Frases, Susana ; Park, Yoon Dong ; Djordjevic, Julianne T. ; Casadevall, Arturo ; Williamson, Peter R. / Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. In: Molecular Microbiology. 2009 ; Vol. 71, No. 5. pp. 1165-1176.
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