Seasonal variation in pituitary-gonadal function in free-ranging impala (Aepyceros melampus)

J. L. Brown, D. E. Wildt, J. R. Raath, V. De Vos, D. L. Janssen, S. B. Citino, J. G. Howard, M. Bush

Research output: Contribution to journalArticle

Abstract

Blood, testicular biopsies and electroejaculates were collected from adult male impala, free-ranging in the Kruger National Park (Republic of South Africa), during the breeding (rut; April-May) and nonbreeding (September-October) seasons. Blood samples were collected at 5-min intervals for 120 min from anaesthetized males (n = 7 impala/group) treated intravenously with saline, gonadotrophin-releasing hormone (GnRH: μg/kg body weight) or human chorionic gonadotrophin (hCG: 10 or 30 iu/kg). Semen was collected from six more animals during the breeding season and 12 animals during the nonbreeding season using a standardized electroejaculation protocol. Ejaculates obtained during the nonbreeding season were of inferior quality to those collected during the breeding season, and were characterized by lower sperm concentrations, poorer sperm motility and more morphologically abnormal sperm forms. Within season, there were no differences in testosterone secretion between the two hCG doses, and these responses were similar to those observed after GnRH, but during the rut, testosterone secretion stimulated by both GnRH and hCG was approximately nine times greater than during the nonbreeding season. This seasonal increase in testosterone production was associated with a doubling in testicular volume and concentrations of luteinizing hormone (LH) receptors. Although concentrations of testicular follicle-stimulating hormone (FSH) receptors were similar between seasons, receptor content increased during rut as a result of increased testicular volume. In contrast to testosterone secretion, basal LH and FSH secretions were unaffected by season and GnRH-induced gonadotrophin secretion was reduced during rut. These data indicate that: (i) seminal quality in free-ranging impala undergoes seasonal changes coincident with alterations in testicular steroidogenic activity, and (ii) the increased testosterone secretion observed during rut is associated with increased testicular sensitivity to LH (via increased gonadotrophin receptors) rather than to increased circulating gonadotrophin concentrations or pituitary responsiveness to GnRH.

Original languageEnglish (US)
Pages (from-to)497-505
Number of pages9
JournalJournal of Reproduction and Fertility
Volume93
Issue number2
DOIs
StatePublished - Jan 1 1991

Keywords

  • FSH
  • Impala
  • LH
  • Receptors
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology

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