Search for viral nucleic acid sequences in brain tissues of patients with schizophrenia using nested polymerase chain reaction

Alla M. Taller, David M. Asher, Kitty L. Pomeroy, Basil A. Eldadah, Mark S. Godec, Peter C. Falkai, Bernhard Bogert, Joel E. Kleinman, Janice R. Stevens, E. Fuller Torrey

Research output: Contribution to journalArticlepeer-review


Background: We used polymerase chain reaction to search for nucleic acid sequences of several viruses in DNA and RNA extracted from brain tissues of schizophrenic and control subjects. Methods: We extracted DNA and RNA templates from frozen brain specimens of 31 patients with schizophrenia and 23 nonschizophrenic control patients with other diseases. The extracts were subjected to polymerase chain reaction with oligonucleotide primers for 12 different viruses (cytomegalovirus, Epstein-Barr virus, herpes simplex virus type I, human herpesvirus type 6, varicella-zoster virus, measles virus, mumps virus, rubella virus, tire picornavirus group, influenza A virus, human T-cell lymphotropic virus type I, and St Louis encephalitis virus), several of which have been suspected of involvement in schizophrenia. Nested primers were used to increase the sensitivity of the method. Results: No amplified nucleic acid sequences encoded by the selected viral genomes were detected in extracts of any brain specimens from either schizophrenic or control patients. Conclusions: These data agree with previous studies that failed to find sequences of a number of viruses in the cerebrospinal fluid or selected areas of the brains of schizophrenic patients. Additional efforts should be undertaken to identify other known and unknown pathogens in schizophrenia, sampling more areas of the brain from subjects with a variety of clinical types of schizophrenia.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalArchives of general psychiatry
Issue number1
StatePublished - Jan 1996
Externally publishedYes

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Psychiatry and Mental health


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