Schwann cell apoptosis in experimental autoimmune neuritis of the Lewis rat and the functional role of tumor necrosis factor-α

Andreas Weishaupt, Wolfgang Brück, Thomas Hartung, Klaus V. Toyka, Ralf Gold

Research output: Contribution to journalArticle

Abstract

Schwann cell (SC) apoptosis may be a critical factor challenging nerve remyelination and regeneration in experimental autoimmune neuritis (EAN) in the Lewis rat. We therefore analyzed the fate of SC during high-dose antigen therapy of adoptive transfer-(AT-) EAN using rhP2 protein. P2 antigen therapy was associated with an increase of tumor necrosis factor (serum levels 1 h after intravenous (i.v.) injection and an augmentation of T-cell apoptosis. Antigen specific therapy had no clear effect on SC apoptosis. The effects on SC apoptosis were determined by morphological criteria or by in situ tailing (IST) followed by immunocytochemical analysis. Secondly, we neutralized TNF-α, released in abundance by antigen treatment but only in small concentrations during natural disease course. We found that the addition of a TNF-α neutralizing antiserum resulted in a significant decrease in the rate of SC apoptosis in vivo compared to animals treated with control antigen rhP0 or with rhP2 only.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalNeuroscience Letters
Volume306
Issue number1-2
DOIs
StatePublished - Jun 22 2001
Externally publishedYes

Keywords

  • Apoptosis
  • Experimental autoimmune neuritis
  • Guillain-Barré syndrome
  • Immunotherapy
  • In situ tailing
  • Peripheral nervous system
  • Schwann cells
  • T-lymphocytes

ASJC Scopus subject areas

  • Neuroscience(all)

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