Schizosaccharomyces pombe rad23 is allelic with swi10, a mating-type switching/radioresistance gene that shares sequence homology with human and mouse ERCC1

Haiying Hang, David N. Hager, Lakshmi Goriparthi, Kevin M. Hopkins, Henry Shih, Howard B. Lieberman

Research output: Contribution to journalArticlepeer-review

Abstract

Schizosaccharomyces pombe (Sp) rad23-1 mutant cells are extremely sensitive to W light and ionizing radiation. A genomic DNA fragment that contains wild-type (wt) rad23 has been cloned. The DNA sequence of this cloned gene has been determined and was found to be identical to the previously characterized mating-type switching/radioresistance gene, swi10. Complementation tests between rad23-1 and swi10-154 mutant cells exclusively produce UV-sensitive progeny and confirm that these two genes are allelic. The DNA sequences of rad23-1 and swi10-154 reveal that each contains a single, unique point mutation. In rad23-1, Glu231 changes to a stop codon, resulting in the production of a truncated protein. In swi10-154, a G to A transition mutation is within a splice consensus sequence for intron 1. Therefore, the corresponding mRNA is incapable of being processed appropriately.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalGene
Volume170
Issue number1
DOIs
StatePublished - Apr 17 1996
Externally publishedYes

Keywords

  • Cloning
  • Ionizing radiation
  • Point mutation
  • Radiation sensitivity
  • Ultraviolet light

ASJC Scopus subject areas

  • Genetics

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