Abstract
Schizosaccharomyces pombe (Sp) rad23-1 mutant cells are extremely sensitive to W light and ionizing radiation. A genomic DNA fragment that contains wild-type (wt) rad23 has been cloned. The DNA sequence of this cloned gene has been determined and was found to be identical to the previously characterized mating-type switching/radioresistance gene, swi10. Complementation tests between rad23-1 and swi10-154 mutant cells exclusively produce UV-sensitive progeny and confirm that these two genes are allelic. The DNA sequences of rad23-1 and swi10-154 reveal that each contains a single, unique point mutation. In rad23-1, Glu231 changes to a stop codon, resulting in the production of a truncated protein. In swi10-154, a G to A transition mutation is within a splice consensus sequence for intron 1. Therefore, the corresponding mRNA is incapable of being processed appropriately.
Original language | English (US) |
---|---|
Pages (from-to) | 113-117 |
Number of pages | 5 |
Journal | Gene |
Volume | 170 |
Issue number | 1 |
DOIs | |
State | Published - Apr 17 1996 |
Externally published | Yes |
Keywords
- Cloning
- Ionizing radiation
- Point mutation
- Radiation sensitivity
- Ultraviolet light
ASJC Scopus subject areas
- Genetics