Schizosaccharomyces pombe Noc3 is essential for ribosome biogenesis and cell division but not DNA replication

Christopher R. Houchens, Audrey Perreault, François Bachand, Thomas Kelly

Research output: Contribution to journalArticle

Abstract

The initiation of eukaryotic DNA replication is preceded by the assembly of prereplication complexes (pre-RCs) at chromosomal origins of DNA replication. Pre-RC assembly requires the essential DNA replication proteins ORC, Cdc6, and Cdt1 to load the MCM DNA helicase onto chromatin. Saccharomyces cerevisiae Noc3 (ScNoc3), an evolutionarily conserved protein originally implicated in 60S ribosomal subunit trafficking, has been proposed to be an essential regulator of DNA replication that plays a direct role during pre-RC formation in budding yeast. We have cloned Schizosaccharomyces pombe noc3+ (Spnoc3 +), the S. pombe homolog of the budding yeast ScNOC3 gene, and functionally characterized the requirement for the SpNoc3 protein during ribosome biogenesis, cell cycle progression, and DNA replication in fission yeast. We showed that fission yeast SpNoc3 is a functional homolog of budding yeast ScNoc3 that is essential for cell viability and ribosome biogenesis. We also showed that SpNoc3 is required for the normal completion of cell division in fission yeast. However, in contrast to the proposal that ScNoc3 plays an essential role during DNA replication in budding yeast, we demonstrated that fission yeast cells do enter and complete S phase in the absence of SpNoc3, suggesting that SpNoc3 is not essential for DNA replication in fission yeast.

Original languageEnglish (US)
Pages (from-to)1433-1440
Number of pages8
JournalEukaryotic Cell
Volume7
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

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Schizosaccharomyces
Schizosaccharomyces pombe
DNA replication
ribosomes
DNA Replication
Ribosomes
Cell Division
cell division
Saccharomycetales
yeasts
Saccharomyces cerevisiae
Eukaryotic Large Ribosome Subunits
DNA helicases
DNA Helicases
Proteins
Replication Origin
protein requirement
biogenesis
S Phase
interphase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Schizosaccharomyces pombe Noc3 is essential for ribosome biogenesis and cell division but not DNA replication. / Houchens, Christopher R.; Perreault, Audrey; Bachand, François; Kelly, Thomas.

In: Eukaryotic Cell, Vol. 7, No. 9, 09.2008, p. 1433-1440.

Research output: Contribution to journalArticle

Houchens, Christopher R. ; Perreault, Audrey ; Bachand, François ; Kelly, Thomas. / Schizosaccharomyces pombe Noc3 is essential for ribosome biogenesis and cell division but not DNA replication. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 9. pp. 1433-1440.
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