Scatter imaging of injured brain slices: detection of mitochondrial injury

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Stroke has been shown to cause exitotoxic injury, two of its manifestations being cellular and mitochondrial swelling. In vitro models of stroke attempt to reproduce the effects of stroke by treating brain tissue with exitotoxins or hypotonic solutions. To further resolve the mechanisms of stroke injury, we have designed a dual-angle scatter imaging (DASI) system sensitive to particle size. The DASI system has been used with a hippocampal slice preparation to contrast cellular swelling, induced by hypotonicity, and combined cellular and mitochondrial swelling caused by excitotoxicity. We found that both hypotonic end excitotoxic treatments caused changes in light scatter. However, only excitotoxic treatment caused a significant change in DASI.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages18-27
Number of pages10
ISBN (Print)0819430749
StatePublished - Jan 1 1999
EventProceedings of the 1999 Optical Diagnostics of Living cells - San Jose, CA, USA
Duration: Jan 25 1999Jan 26 1999

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume3604
ISSN (Print)0277-786X

Other

OtherProceedings of the 1999 Optical Diagnostics of Living cells
CitySan Jose, CA, USA
Period1/25/991/26/99

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Johnson, L., Hanley, D., & Thakor, N. (1999). Scatter imaging of injured brain slices: detection of mitochondrial injury. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 18-27). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 3604). Society of Photo-Optical Instrumentation Engineers.