TY - GEN
T1 - Scatter imaging of injured brain slices
T2 - Proceedings of the 1999 Optical Diagnostics of Living cells
AU - Johnson, Lee
AU - Hanley, Daniel
AU - Thakor, Nitish
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Stroke has been shown to cause exitotoxic injury, two of its manifestations being cellular and mitochondrial swelling. In vitro models of stroke attempt to reproduce the effects of stroke by treating brain tissue with exitotoxins or hypotonic solutions. To further resolve the mechanisms of stroke injury, we have designed a dual-angle scatter imaging (DASI) system sensitive to particle size. The DASI system has been used with a hippocampal slice preparation to contrast cellular swelling, induced by hypotonicity, and combined cellular and mitochondrial swelling caused by excitotoxicity. We found that both hypotonic end excitotoxic treatments caused changes in light scatter. However, only excitotoxic treatment caused a significant change in DASI.
AB - Stroke has been shown to cause exitotoxic injury, two of its manifestations being cellular and mitochondrial swelling. In vitro models of stroke attempt to reproduce the effects of stroke by treating brain tissue with exitotoxins or hypotonic solutions. To further resolve the mechanisms of stroke injury, we have designed a dual-angle scatter imaging (DASI) system sensitive to particle size. The DASI system has been used with a hippocampal slice preparation to contrast cellular swelling, induced by hypotonicity, and combined cellular and mitochondrial swelling caused by excitotoxicity. We found that both hypotonic end excitotoxic treatments caused changes in light scatter. However, only excitotoxic treatment caused a significant change in DASI.
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M3 - Conference contribution
AN - SCOPUS:0032675180
SN - 0819430749
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 18
EP - 27
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Society of Photo-Optical Instrumentation Engineers
Y2 - 25 January 1999 through 26 January 1999
ER -