Scanning electron microscopy and freeze etching of gonorrhoeal urethral exudate

H. Farzadegan, I. L. Roth

Research output: Contribution to journalArticlepeer-review

Abstract

Urethral exudates from male patients with gonorrhoea were studied by use of the critical point drying technique for scanning electron microscopy. The technique for scanning electron microscopy. The surfaces of phagocytes were extensively ruffled. This was interpreted to be the 3 dimensional appearance of pseudopodia. Epithelial cells present in the urethral exudate showed a highly convoluted surface structure. Specimens of urethral exudate prepared by the freeze etch technique showed that most N. gonorrhoeae were engulfed individually in phagocytes. Some phagosomes contained 2 or more gonococcal cells. Phagocytes containing gonococci were degranulated, but small granules were present in the phagosomes. Nuclear pores on the surface of the nuclear membrane were always associated with an indentation of the nuclear membrane. The outer layer of the nuclear membrane was smooth compared to the inner layer.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalBRIT.J.VENER.DIS.
Volume51
Issue number2
DOIs
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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