Scanning cytophotometric analysis of myocardial nucleic acid and chromatin changes in soman toxicated rabbits

Lee J. Martin, Jeffrey A. Doebler, John W. Swisher, Adam Anthony, Tsung‐Ming ‐M Shih

Research output: Contribution to journalArticle

Abstract

Myocardial nucleic acid responses were analysed in New Zealand White rabbits 20 min–1 h and 6–8 h following single subcutaneous injections of soman (20, 30, or 40 μg kg−1). Scanning‐integrating microdensitometry was used to quantify Azure B‐RNA and Feulgen–DNA (F‐DNA) levels, and changes in the suseptibility of chromatin to Feulgen acid hydrolysis (F‐DNA reactivity) of individual ventricular myocardial cells. With a dosage of 20 μg kg−1 soman, no RNA alterations were evidenced at 1 h whereas at 6–8 h myocardial cells exhibited higher RNA levels and an increase in F‐DNA reactivity of chromatin. With dosages of 30 and 40 μg kg−1 soman there was an augmentation in RNA levels and in the acid hydrolysability of nuclear chromatin at both 20 min–1 h and 6–8 h. It is postulated that the observed cellular transformations represent a compensatory augmentation in myocardial metabolic functioning presumably in response to an increased functional demand on the ventricular myocardium. The absence of cytopathic or cytochemical evidence of impairment in nucleic acid metabolism is inconsistent with the premise that soman exerts direct cytotoxic effects on rabbit myocardium.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalCell Biochemistry and Function
Volume2
Issue number4
DOIs
StatePublished - Oct 1984
Externally publishedYes

Keywords

  • Azure B‐RNA
  • Feulgen–DNA
  • Heart
  • cardiac muscle
  • cytochemistry
  • microdensitometry
  • soman toxicity

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Scanning cytophotometric analysis of myocardial nucleic acid and chromatin changes in soman toxicated rabbits'. Together they form a unique fingerprint.

  • Cite this