Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues

Yicong Wu, Yuxin Leng, Jiefeng Xi, Xingde Li

Research output: Contribution to journalArticle

Abstract

An extremely compact all-fiber-optic scanning endomicroscopy system was developed for two-photon fluorescence (TPF) and second harmonic generation (SHG) imaging of biological samples. A conventional double-clad fiber (DCF) was employed in the endomicroscope for single-mode femtosecond pulse delivery, multimode nonlinear optical signals collection and fast two-dimensional scanning. A single photonic bandgap fiber (PBF) with negative group velocity dispersion at two-photon excitation wavelength (i.e. ∼810 nm) was used for pulse prechirping in replacement of a bulky grating/lens-based pulse stretcher. The combined use of DCF and PBF in the endomicroscopy system made the endomicroscope basically a plug-and-play unit. The excellent imaging ability of the extremely compact all-fiber-optic nonlinear optical endomicroscopy system was demonstrated by SHG imaging of rat tail tendon and depth-resolved TPF imaging of epithelial tissues stained with acridine orange. The preliminary results suggested the promising potential of this extremely compact all-fiber-optic endomicroscopy system for realtime assessment of both epithelial and stromal structures in luminal organs.

Original languageEnglish (US)
Pages (from-to)7907-7915
Number of pages9
JournalOptics Express
Volume17
Issue number10
DOIs
StatePublished - May 11 2009

Fingerprint

fiber optics
scanning
fibers
harmonic generations
photons
pulses
photonics
tendons
fluorescence
plugs
group velocity
organs
rats
optical communication
delivery
lenses
gratings
wavelengths
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues. / Wu, Yicong; Leng, Yuxin; Xi, Jiefeng; Li, Xingde.

In: Optics Express, Vol. 17, No. 10, 11.05.2009, p. 7907-7915.

Research output: Contribution to journalArticle

Wu, Yicong ; Leng, Yuxin ; Xi, Jiefeng ; Li, Xingde. / Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues. In: Optics Express. 2009 ; Vol. 17, No. 10. pp. 7907-7915.
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