Saturable retention of vasopressin by hippocampus vessels in vivo, associated with inhibition of blood-brain transfer of large neutral amino acids

J. Reith, A. Ermisch, N. H. Diemer, A. Gjedde

Research output: Contribution to journalArticle

Abstract

Vasopressin receptors have been reported in the endothelium of brain capillaries. The function of these receptors is not known. To test the prediction that vasopressin receptors in brain capillary endothelium affect amino acid transport across the blood-brain barrier and to assess the role of vasopressin transport across the cerebral vascular endothelium, we measured (a) the endothelial permeability to the large neutral amino acid leucine in the absence and presence of arginine vasopressin (AVP) and (b) the permeability of the blood-brain barrier to AVP relative to mannitol. In brain regions protected by the blood-brain barrier, after circulation for 20 s, coinjection of leucine and AVP intravenously led to a decrease of leucine transport unrelated to changes of blood flow. The decrease was most pronounced in hippocampus (42%) and least pronounced in olfactory bulb and colliculi (17 and 19%, respectively). In the latter regions, the endothelial permeability to AVP did not significantly exceed that of mannitol. In hippocampus and in regions with no blood-brain barrier (pituitary and pineal glands), AVP retention in excess of mannitol retention was blocked by unlabeled AVP. The findings do not contradict the hypothesis of a role for AVP in the regulation of large neutral amino acid transfer into brain tissue.

Original languageEnglish (US)
Pages (from-to)1471-1479
Number of pages9
JournalJournal of Neurochemistry
Volume49
Issue number5
StatePublished - 1987
Externally publishedYes

Fingerprint

Neutral Amino Acids
Arginine Vasopressin
Vasopressins
Hippocampus
Brain
Blood
Blood-Brain Barrier
Vascular Endothelium
Mannitol
Leucine
Vasopressin Receptors
Permeability
Pineal Gland
Olfactory Bulb
Pituitary Gland
Tissue
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Saturable retention of vasopressin by hippocampus vessels in vivo, associated with inhibition of blood-brain transfer of large neutral amino acids. / Reith, J.; Ermisch, A.; Diemer, N. H.; Gjedde, A.

In: Journal of Neurochemistry, Vol. 49, No. 5, 1987, p. 1471-1479.

Research output: Contribution to journalArticle

@article{33d0d653591e42f8aa520b360bfa9e24,
title = "Saturable retention of vasopressin by hippocampus vessels in vivo, associated with inhibition of blood-brain transfer of large neutral amino acids",
abstract = "Vasopressin receptors have been reported in the endothelium of brain capillaries. The function of these receptors is not known. To test the prediction that vasopressin receptors in brain capillary endothelium affect amino acid transport across the blood-brain barrier and to assess the role of vasopressin transport across the cerebral vascular endothelium, we measured (a) the endothelial permeability to the large neutral amino acid leucine in the absence and presence of arginine vasopressin (AVP) and (b) the permeability of the blood-brain barrier to AVP relative to mannitol. In brain regions protected by the blood-brain barrier, after circulation for 20 s, coinjection of leucine and AVP intravenously led to a decrease of leucine transport unrelated to changes of blood flow. The decrease was most pronounced in hippocampus (42{\%}) and least pronounced in olfactory bulb and colliculi (17 and 19{\%}, respectively). In the latter regions, the endothelial permeability to AVP did not significantly exceed that of mannitol. In hippocampus and in regions with no blood-brain barrier (pituitary and pineal glands), AVP retention in excess of mannitol retention was blocked by unlabeled AVP. The findings do not contradict the hypothesis of a role for AVP in the regulation of large neutral amino acid transfer into brain tissue.",
author = "J. Reith and A. Ermisch and Diemer, {N. H.} and A. Gjedde",
year = "1987",
language = "English (US)",
volume = "49",
pages = "1471--1479",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Saturable retention of vasopressin by hippocampus vessels in vivo, associated with inhibition of blood-brain transfer of large neutral amino acids

AU - Reith, J.

AU - Ermisch, A.

AU - Diemer, N. H.

AU - Gjedde, A.

PY - 1987

Y1 - 1987

N2 - Vasopressin receptors have been reported in the endothelium of brain capillaries. The function of these receptors is not known. To test the prediction that vasopressin receptors in brain capillary endothelium affect amino acid transport across the blood-brain barrier and to assess the role of vasopressin transport across the cerebral vascular endothelium, we measured (a) the endothelial permeability to the large neutral amino acid leucine in the absence and presence of arginine vasopressin (AVP) and (b) the permeability of the blood-brain barrier to AVP relative to mannitol. In brain regions protected by the blood-brain barrier, after circulation for 20 s, coinjection of leucine and AVP intravenously led to a decrease of leucine transport unrelated to changes of blood flow. The decrease was most pronounced in hippocampus (42%) and least pronounced in olfactory bulb and colliculi (17 and 19%, respectively). In the latter regions, the endothelial permeability to AVP did not significantly exceed that of mannitol. In hippocampus and in regions with no blood-brain barrier (pituitary and pineal glands), AVP retention in excess of mannitol retention was blocked by unlabeled AVP. The findings do not contradict the hypothesis of a role for AVP in the regulation of large neutral amino acid transfer into brain tissue.

AB - Vasopressin receptors have been reported in the endothelium of brain capillaries. The function of these receptors is not known. To test the prediction that vasopressin receptors in brain capillary endothelium affect amino acid transport across the blood-brain barrier and to assess the role of vasopressin transport across the cerebral vascular endothelium, we measured (a) the endothelial permeability to the large neutral amino acid leucine in the absence and presence of arginine vasopressin (AVP) and (b) the permeability of the blood-brain barrier to AVP relative to mannitol. In brain regions protected by the blood-brain barrier, after circulation for 20 s, coinjection of leucine and AVP intravenously led to a decrease of leucine transport unrelated to changes of blood flow. The decrease was most pronounced in hippocampus (42%) and least pronounced in olfactory bulb and colliculi (17 and 19%, respectively). In the latter regions, the endothelial permeability to AVP did not significantly exceed that of mannitol. In hippocampus and in regions with no blood-brain barrier (pituitary and pineal glands), AVP retention in excess of mannitol retention was blocked by unlabeled AVP. The findings do not contradict the hypothesis of a role for AVP in the regulation of large neutral amino acid transfer into brain tissue.

UR - http://www.scopus.com/inward/record.url?scp=0023230534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023230534&partnerID=8YFLogxK

M3 - Article

C2 - 2959752

AN - SCOPUS:0023230534

VL - 49

SP - 1471

EP - 1479

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 5

ER -