SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy

Harini Natarajan; Andrew R. Crowley; Savannah E. Butler; Shiwei Xu; Joshua A. Weiner; Evan M. Bloch; Kirsten Littlefield; Wendy Wieland-Alter; Ruth I. Connor; Peter F. Wright; Sarah E. Benner; Tania S. Bonny; Oliver Laeyendecker; David Sullivan; Shmuel Shoham; Thomas C. Quinn; Harry Larman; Arturo Casadevall; Andrew Pekosz; Andrew D. Redd; Aaron A.R. Tobian; Margaret E. Ackerman

doi:10.1101/2020.09.16.20196154

SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy

Harini Natarajan, Andrew R. Crowley, Savannah E. Butler, Shiwei Xu, Joshua A. Weiner, Evan M. Bloch, Kirsten Littlefield, Wendy Wieland-Alter, Ruth I. Connor, Peter F. Wright, Sarah E. Benner, Tania S. Bonny, Oliver Laeyendecker, David Sullivan, Shmuel Shoham, Thomas C. Quinn, Harry Larman, Arturo Casadevall, Andrew Pekosz, Andrew D. ReddAaron A.R. Tobian, Margaret E. Ackerman

Research output: Contribution to journal › Article › peer-review

Abstract

Convalescent plasma has emerged as a promising COVID-19 treatment. However, the humoral factors that contribute to efficacy are poorly understood. This study functionally and phenotypically profiled plasma from eligible convalescent donors. In addition to viral neutralization, convalescent plasma contained antibodies capable of mediating such Fc-dependent functions as complement activation, phagocytosis and antibody-dependent cellular cytotoxicity against SARS-CoV-2. These activities expand the antiviral functions associated with convalescent plasma and together with neutralization efficacy, could be accurately and robustly from antibody phenotypes. These results suggest that high-throughput profiling could be used to screen donors and plasma may provide benefits beyond neutralization.

Original language	English (US)
Journal	Unknown Journal
DOIs	https://doi.org/10.1101/2020.09.16.20196154
State	Published - Sep 18 2020

Keywords

ADCC
Convalescent plasma
COVID-19
Functional antibody response
Neutralization
Phagocytosis
SARS-CoV-2

ASJC Scopus subject areas

Medicine(all)

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Natarajan, H., Crowley, A. R., Butler, S. E., Xu, S., Weiner, J. A., Bloch, E. M., Littlefield, K., Wieland-Alter, W., Connor, R. I., Wright, P. F., Benner, S. E., Bonny, T. S., Laeyendecker, O., Sullivan, D., Shoham, S., Quinn, T. C., Larman, H., Casadevall, A., Pekosz, A., ... Ackerman, M. E. (2020). SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy. Unknown Journal. https://doi.org/10.1101/2020.09.16.20196154

SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy. / Natarajan, Harini; Crowley, Andrew R.; Butler, Savannah E.; Xu, Shiwei; Weiner, Joshua A.; Bloch, Evan M.; Littlefield, Kirsten; Wieland-Alter, Wendy; Connor, Ruth I.; Wright, Peter F.; Benner, Sarah E.; Bonny, Tania S.; Laeyendecker, Oliver ; Sullivan, David ; Shoham, Shmuel ; Quinn, Thomas C.; Larman, Harry ; Casadevall, Arturo ; Pekosz, Andrew ; Redd, Andrew D.; Tobian, Aaron A.R.; Ackerman, Margaret E.

In: Unknown Journal, 18.09.2020.

Research output: Contribution to journal › Article › peer-review

Natarajan, H, Crowley, AR, Butler, SE, Xu, S, Weiner, JA, Bloch, EM, Littlefield, K, Wieland-Alter, W, Connor, RI, Wright, PF, Benner, SE, Bonny, TS, Laeyendecker, O , Sullivan, D , Shoham, S , Quinn, TC , Larman, H , Casadevall, A , Pekosz, A , Redd, AD , Tobian, AAR & Ackerman, ME 2020, 'SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy', Unknown Journal. https://doi.org/10.1101/2020.09.16.20196154

Natarajan, Harini ; Crowley, Andrew R. ; Butler, Savannah E. ; Xu, Shiwei ; Weiner, Joshua A. ; Bloch, Evan M. ; Littlefield, Kirsten ; Wieland-Alter, Wendy ; Connor, Ruth I. ; Wright, Peter F. ; Benner, Sarah E. ; Bonny, Tania S. ; Laeyendecker, Oliver ; Sullivan, David ; Shoham, Shmuel ; Quinn, Thomas C. ; Larman, Harry ; Casadevall, Arturo ; Pekosz, Andrew ; Redd, Andrew D. ; Tobian, Aaron A.R. ; Ackerman, Margaret E. / SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy. In: Unknown Journal. 2020.

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abstract = "Convalescent plasma has emerged as a promising COVID-19 treatment. However, the humoral factors that contribute to efficacy are poorly understood. This study functionally and phenotypically profiled plasma from eligible convalescent donors. In addition to viral neutralization, convalescent plasma contained antibodies capable of mediating such Fc-dependent functions as complement activation, phagocytosis and antibody-dependent cellular cytotoxicity against SARS-CoV-2. These activities expand the antiviral functions associated with convalescent plasma and together with neutralization efficacy, could be accurately and robustly from antibody phenotypes. These results suggest that high-throughput profiling could be used to screen donors and plasma may provide benefits beyond neutralization.",

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AU - Tobian, Aaron A.R.

AU - Ackerman, Margaret E.

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