Abstract
Background: Convalescent plasma therapy is a leading treatment for conferring temporary immunity to COVID-19-susceptible individuals or for use as post-exposure prophylaxis. However, not all recovered patients develop adequate antibody titers for donation and the relationship between avidity and neutralizing titers is currently not well understood. Methods: SARS-CoV-2 anti-spike and anti-nucleocapsid IgG titers and avidity were measured in a longitudinal cohort of COVID-19 hospitalized patients (n= 16 individuals) and a cross-sectional sample of convalescent plasma donors (n =130). Epidemiologic correlates of avidity were examined in donors by linear regression. The association of avidity and a high neutralizing titer (NT) were also assessed in donors using modified Poisson regression. Results: Antibody avidity increased over duration of infection and remained elevated. In convalescent plasma donors, higher levels of anti-spike avidity were associated with older age, male sex, and hospitalization. Higher NTs had a stronger positive correlation with anti-spike IgG avidity (Spearman ρ = 0.386; P<.001) than with anti-nucleocapsid IgG avidity (Spearman ρ = 0.211; P=.026). Increasing levels of anti-spike IgG avidity were associated with high NT (≥160) (adjusted prevalence ratio = 1.58 [95% confidence interval=1.19-2.12]), independent of age, sex, and hospitalization. Conclusions: SARS-CoV-2 antibody avidity correlated with duration of infection and higher neutralizing titers, suggesting a potential alternative screening parameter for identifying optimal convalescent plasma donors.
Original language | English (US) |
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Pages (from-to) | 1974-1984 |
Number of pages | 11 |
Journal | Journal of Infectious Diseases |
Volume | 222 |
Issue number | 12 |
DOIs | |
State | Published - Dec 15 2020 |
Keywords
- Anti-nucleocapsid
- Anti-spike
- Avidity
- Convalescent plasma
- SARS-CoV-2
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases