Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever

Alaullah Sheikh, M. Saruar Bhuiyan, Farhana Khanam, Fahima Chowdhury, Amit Saha, Dilruba Ahmed, K. M A Jamil, Regina C. LaRocque, Jason B. Harris, Mian Mashhud Ahmad, Richelle Charles, W Abdullah Brooks, Stephen B. Calderwood, Alejandro Cravioto, Edward T. Ryan, Firdausi Qadri

Research output: Contribution to journalArticle

Abstract

Many currently available diagnostic tests for typhoid fever lack sensitivity and/or specificity, especially in areas of the world where the disease is endemic. In order to identify a diagnostic test that better correlates with typhoid fever, we evaluated immune responses to Salmonella enterica serovar Typhi (serovar Typhi) in individuals with suspected typhoid fever in Dhaka, Bangladesh. We enrolled 112 individuals with suspected typhoid fever, cultured day 0 blood for serovar Typhi organisms, and performed Widal assays on days 0, 5, and 20. We harvested peripheral blood lymphocytes and analyzed antibody levels in supernatants collected on days 0, 5, and 20 (using an antibody-in-lymphocyte-supernatant [ALS] assay), as well as in plasma on these days. We measured ALS reactivity to a serovar Typhi membrane preparation (MP), a formalin-inactivated whole-cell preparation, and serovar Typhi lipopolysaccharide. We measured responses in healthy Bangladeshi, as well as in Bangladeshi febrile patients with confirmed dengue fever or leptospirosis. We categorized suspected typhoid fever individuals into different groups (groups I to V) based on blood culture results, Widal titer, and clinical features. Responses to MP antigen in the immunoglobulin A isotype were detectable at the time of presentation in the plasma of 81% of patients. The ALS assay, however, tested positive in all patients with documented or highly suspicious typhoid, suggesting that such a response could be the basis of improved diagnostic point-of-care-assay for serovar Typhi infection. It can be important for use in epidemiological studies, as well as in difficult cases involving fevers of unknown origin.

Original languageEnglish (US)
Pages (from-to)1587-1594
Number of pages8
JournalClinical and Vaccine Immunology
Volume16
Issue number11
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Salmonella typhi
Salmonella
Lymphocytes
Typhoid Fever
Immunoglobulin A
Antibody Formation
Assays
Plasmas
Blood
Antibodies
Membranes
Routine Diagnostic Tests
Point-of-Care Systems
Formaldehyde
Fever of Unknown Origin
Lipopolysaccharides
Endemic Diseases
Leptospirosis
Bangladesh
Dengue

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever. / Sheikh, Alaullah; Bhuiyan, M. Saruar; Khanam, Farhana; Chowdhury, Fahima; Saha, Amit; Ahmed, Dilruba; Jamil, K. M A; LaRocque, Regina C.; Harris, Jason B.; Ahmad, Mian Mashhud; Charles, Richelle; Brooks, W Abdullah; Calderwood, Stephen B.; Cravioto, Alejandro; Ryan, Edward T.; Qadri, Firdausi.

In: Clinical and Vaccine Immunology, Vol. 16, No. 11, 11.2009, p. 1587-1594.

Research output: Contribution to journalArticle

Sheikh, A, Bhuiyan, MS, Khanam, F, Chowdhury, F, Saha, A, Ahmed, D, Jamil, KMA, LaRocque, RC, Harris, JB, Ahmad, MM, Charles, R, Brooks, WA, Calderwood, SB, Cravioto, A, Ryan, ET & Qadri, F 2009, 'Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever', Clinical and Vaccine Immunology, vol. 16, no. 11, pp. 1587-1594. https://doi.org/10.1128/CVI.00311-09
Sheikh, Alaullah ; Bhuiyan, M. Saruar ; Khanam, Farhana ; Chowdhury, Fahima ; Saha, Amit ; Ahmed, Dilruba ; Jamil, K. M A ; LaRocque, Regina C. ; Harris, Jason B. ; Ahmad, Mian Mashhud ; Charles, Richelle ; Brooks, W Abdullah ; Calderwood, Stephen B. ; Cravioto, Alejandro ; Ryan, Edward T. ; Qadri, Firdausi. / Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever. In: Clinical and Vaccine Immunology. 2009 ; Vol. 16, No. 11. pp. 1587-1594.
@article{117319260f3f4df59891780d8eb2f0af,
title = "Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever",
abstract = "Many currently available diagnostic tests for typhoid fever lack sensitivity and/or specificity, especially in areas of the world where the disease is endemic. In order to identify a diagnostic test that better correlates with typhoid fever, we evaluated immune responses to Salmonella enterica serovar Typhi (serovar Typhi) in individuals with suspected typhoid fever in Dhaka, Bangladesh. We enrolled 112 individuals with suspected typhoid fever, cultured day 0 blood for serovar Typhi organisms, and performed Widal assays on days 0, 5, and 20. We harvested peripheral blood lymphocytes and analyzed antibody levels in supernatants collected on days 0, 5, and 20 (using an antibody-in-lymphocyte-supernatant [ALS] assay), as well as in plasma on these days. We measured ALS reactivity to a serovar Typhi membrane preparation (MP), a formalin-inactivated whole-cell preparation, and serovar Typhi lipopolysaccharide. We measured responses in healthy Bangladeshi, as well as in Bangladeshi febrile patients with confirmed dengue fever or leptospirosis. We categorized suspected typhoid fever individuals into different groups (groups I to V) based on blood culture results, Widal titer, and clinical features. Responses to MP antigen in the immunoglobulin A isotype were detectable at the time of presentation in the plasma of 81{\%} of patients. The ALS assay, however, tested positive in all patients with documented or highly suspicious typhoid, suggesting that such a response could be the basis of improved diagnostic point-of-care-assay for serovar Typhi infection. It can be important for use in epidemiological studies, as well as in difficult cases involving fevers of unknown origin.",
author = "Alaullah Sheikh and Bhuiyan, {M. Saruar} and Farhana Khanam and Fahima Chowdhury and Amit Saha and Dilruba Ahmed and Jamil, {K. M A} and LaRocque, {Regina C.} and Harris, {Jason B.} and Ahmad, {Mian Mashhud} and Richelle Charles and Brooks, {W Abdullah} and Calderwood, {Stephen B.} and Alejandro Cravioto and Ryan, {Edward T.} and Firdausi Qadri",
year = "2009",
month = "11",
doi = "10.1128/CVI.00311-09",
language = "English (US)",
volume = "16",
pages = "1587--1594",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Salmonella enterica serovar typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever

AU - Sheikh, Alaullah

AU - Bhuiyan, M. Saruar

AU - Khanam, Farhana

AU - Chowdhury, Fahima

AU - Saha, Amit

AU - Ahmed, Dilruba

AU - Jamil, K. M A

AU - LaRocque, Regina C.

AU - Harris, Jason B.

AU - Ahmad, Mian Mashhud

AU - Charles, Richelle

AU - Brooks, W Abdullah

AU - Calderwood, Stephen B.

AU - Cravioto, Alejandro

AU - Ryan, Edward T.

AU - Qadri, Firdausi

PY - 2009/11

Y1 - 2009/11

N2 - Many currently available diagnostic tests for typhoid fever lack sensitivity and/or specificity, especially in areas of the world where the disease is endemic. In order to identify a diagnostic test that better correlates with typhoid fever, we evaluated immune responses to Salmonella enterica serovar Typhi (serovar Typhi) in individuals with suspected typhoid fever in Dhaka, Bangladesh. We enrolled 112 individuals with suspected typhoid fever, cultured day 0 blood for serovar Typhi organisms, and performed Widal assays on days 0, 5, and 20. We harvested peripheral blood lymphocytes and analyzed antibody levels in supernatants collected on days 0, 5, and 20 (using an antibody-in-lymphocyte-supernatant [ALS] assay), as well as in plasma on these days. We measured ALS reactivity to a serovar Typhi membrane preparation (MP), a formalin-inactivated whole-cell preparation, and serovar Typhi lipopolysaccharide. We measured responses in healthy Bangladeshi, as well as in Bangladeshi febrile patients with confirmed dengue fever or leptospirosis. We categorized suspected typhoid fever individuals into different groups (groups I to V) based on blood culture results, Widal titer, and clinical features. Responses to MP antigen in the immunoglobulin A isotype were detectable at the time of presentation in the plasma of 81% of patients. The ALS assay, however, tested positive in all patients with documented or highly suspicious typhoid, suggesting that such a response could be the basis of improved diagnostic point-of-care-assay for serovar Typhi infection. It can be important for use in epidemiological studies, as well as in difficult cases involving fevers of unknown origin.

AB - Many currently available diagnostic tests for typhoid fever lack sensitivity and/or specificity, especially in areas of the world where the disease is endemic. In order to identify a diagnostic test that better correlates with typhoid fever, we evaluated immune responses to Salmonella enterica serovar Typhi (serovar Typhi) in individuals with suspected typhoid fever in Dhaka, Bangladesh. We enrolled 112 individuals with suspected typhoid fever, cultured day 0 blood for serovar Typhi organisms, and performed Widal assays on days 0, 5, and 20. We harvested peripheral blood lymphocytes and analyzed antibody levels in supernatants collected on days 0, 5, and 20 (using an antibody-in-lymphocyte-supernatant [ALS] assay), as well as in plasma on these days. We measured ALS reactivity to a serovar Typhi membrane preparation (MP), a formalin-inactivated whole-cell preparation, and serovar Typhi lipopolysaccharide. We measured responses in healthy Bangladeshi, as well as in Bangladeshi febrile patients with confirmed dengue fever or leptospirosis. We categorized suspected typhoid fever individuals into different groups (groups I to V) based on blood culture results, Widal titer, and clinical features. Responses to MP antigen in the immunoglobulin A isotype were detectable at the time of presentation in the plasma of 81% of patients. The ALS assay, however, tested positive in all patients with documented or highly suspicious typhoid, suggesting that such a response could be the basis of improved diagnostic point-of-care-assay for serovar Typhi infection. It can be important for use in epidemiological studies, as well as in difficult cases involving fevers of unknown origin.

UR - http://www.scopus.com/inward/record.url?scp=70350736247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350736247&partnerID=8YFLogxK

U2 - 10.1128/CVI.00311-09

DO - 10.1128/CVI.00311-09

M3 - Article

C2 - 19741090

AN - SCOPUS:70350736247

VL - 16

SP - 1587

EP - 1594

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 11

ER -