Sall1 transiently marks undifferentiated heart precursors and regulates their fate

Yuika Morita, Peter Andersen, Akitsu Hotta, Yuko Tsukahara, Noriko Sasagawa, Naoko Hayashida, Chizuko Koga, Misato Nishikawa, Yumiko Saga, Sylvia M. Evans, Kazuko Koshiba-Takeuchi, Ryuichi Nishinakamura, Yoshinori Yoshida, Chulan Kwon, Jun K. Takeuchi

Research output: Contribution to journalArticlepeer-review

Abstract

Cardiac progenitor cells (CPCs) are a crucial source of cells in cardiac development and regeneration. However, reported CPCs are heterogeneous, and no gene has been identified to transiently mark undifferentiated CPCs throughout heart development. Here we show that Spalt-like gene 1 (Sall1), a zing-finger transcription factor, is expressed in undifferentiated CPCs giving rise to both left and right ventricles. Sall1 was transiently expressed in precardiac mesoderm contributing to the first heart field (left ventricle precursors) but not in the field itself. Similarly, Sall1 expression was maintained in the second heart field (outflow tract/right ventricle precursors) but not in cardiac cells. In vitro, high levels of Sall1 at mesodermal stages enhanced cardiomyogenesis, whereas its continued expression suppressed cardiac differentiation. Our study demonstrates that Sall1 marks CPCs in an undifferentiated state and regulates cardiac differentiation. These findings provide fundamental insights into CPC maintenance, which can be instrumental for CPC-based regenerative medicine.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalJournal of Molecular and Cellular Cardiology
Volume92
DOIs
StatePublished - Mar 1 2016

Keywords

  • Cardiac development
  • Cardiac progenitor
  • Cardiac transcription factors
  • ES/iPS cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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