Salivary gland-specific gene expression in the malaria vector Anopheles gambiae

B. Arcà, F. Lombardo, M. De Lara Capurro, A. Della Torre, L. Spanos, George Dimopoulos, C. Louis, A. A. James, M. Coluzzi

Research output: Contribution to journalArticle

Abstract

Molecular studies on the tissue-specific gene expression in the salivary glands of Anopheles gambiae may provide useful tools for the development of new strategies for the control of the most efficient malaria vector in the sub-Saharan Africa. We summarize here the results of a recent investigation focused on the isolation of secreted factors and putative receptors from the salivary glands of An. gambiae. Using the Signal Sequence Trap technique we have identified the first cDNAs specifically expressed in the An. gambiae salivary glands. Among these, four are exclusively expressed in female glands and encode factors presumably involved in blood-feeding, whereas two other cDNAs seem to be expressed both in male and in female glands and are likely implicated in sugar-feeding. Homologues of genes previously identified in the yellow fever mosquito Aedes aegypti, like the apyrase and D7, as well as novel salivary gland-specific cDNAs, were identified. The isolation and characterization of promoter sequences from the corresponding genes may prove useful for the expression of anti parasitic agents in the salivary glands of transgenic mosquitoes.

Original languageEnglish (US)
Pages (from-to)483-487
Number of pages5
JournalParassitologia
Volume41
Issue number1-3
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Anopheles gambiae
Salivary Glands
Malaria
Gene Expression
Complementary DNA
Culicidae
Apyrase
Yellow Fever
Aedes
Africa South of the Sahara
Protein Sorting Signals
Genes

Keywords

  • Anopheles gambiae salivary glands
  • Apyrase
  • D7
  • D7-related
  • Gambiae salivary gland genes (gsg)
  • Signal sequence trap (sst)

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Arcà, B., Lombardo, F., De Lara Capurro, M., Della Torre, A., Spanos, L., Dimopoulos, G., ... Coluzzi, M. (1999). Salivary gland-specific gene expression in the malaria vector Anopheles gambiae. Parassitologia, 41(1-3), 483-487.

Salivary gland-specific gene expression in the malaria vector Anopheles gambiae. / Arcà, B.; Lombardo, F.; De Lara Capurro, M.; Della Torre, A.; Spanos, L.; Dimopoulos, George; Louis, C.; James, A. A.; Coluzzi, M.

In: Parassitologia, Vol. 41, No. 1-3, 09.1999, p. 483-487.

Research output: Contribution to journalArticle

Arcà, B, Lombardo, F, De Lara Capurro, M, Della Torre, A, Spanos, L, Dimopoulos, G, Louis, C, James, AA & Coluzzi, M 1999, 'Salivary gland-specific gene expression in the malaria vector Anopheles gambiae', Parassitologia, vol. 41, no. 1-3, pp. 483-487.
Arcà B, Lombardo F, De Lara Capurro M, Della Torre A, Spanos L, Dimopoulos G et al. Salivary gland-specific gene expression in the malaria vector Anopheles gambiae. Parassitologia. 1999 Sep;41(1-3):483-487.
Arcà, B. ; Lombardo, F. ; De Lara Capurro, M. ; Della Torre, A. ; Spanos, L. ; Dimopoulos, George ; Louis, C. ; James, A. A. ; Coluzzi, M. / Salivary gland-specific gene expression in the malaria vector Anopheles gambiae. In: Parassitologia. 1999 ; Vol. 41, No. 1-3. pp. 483-487.
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