Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi

Michael B. Wells, Deborah J Andrew

Research output: Contribution to journalArticle

Abstract

Background: Anopheles mosquitoes are vectors for malaria, a disease with continued grave outcomes for human health. Transmission of malaria from mosquitoes to humans occurs by parasite passage through the salivary glands (SGs). Previous studies of mosquito SG architecture have been limited in scope and detail. Methods: We developed a simple, optimized protocol for fluorescence staining using dyes and/or antibodies to interrogate cellular architecture in Anopheles stephensi adult SGs. We used common biological dyes, antibodies to well-conserved structural and organellar markers, and antibodies against Anopheles salivary proteins to visualize many individual SGs at high resolution by confocal microscopy. Results: These analyses confirmed morphological features previously described using electron microscopy and uncovered a high degree of individual variation in SG structure. Our studies provide evidence for two alternative models for the origin of the salivary duct, the structure facilitating parasite transport out of SGs. We compare SG cellular architecture in An. stephensi and Drosophila melanogaster, a fellow Dipteran whose adult SGs are nearly completely unstudied, and find many conserved features despite divergence in overall form and function. Anopheles salivary proteins previously observed at the basement membrane were localized either in SG cells, secretory cavities, or the SG lumen. Our studies also revealed a population of cells with characteristics consistent with regenerative cells, similar to muscle satellite cells or midgut regenerative cells. Conclusions: This work serves as a foundation for linking Anopheles stephensi SG cellular architecture to function and as a basis for generating and evaluating tools aimed at preventing malaria transmission at the level of mosquito SGs.

Original languageEnglish (US)
Article number617
JournalParasites and Vectors
Volume8
Issue number1
DOIs
StatePublished - Dec 2 2015

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Anopheles
Salivary Glands
Malaria
Culicidae
Salivary Proteins and Peptides
Mosquito Vectors
Antibodies
Parasites
Coloring Agents
Salivary Ducts
Drosophila melanogaster
Basement Membrane
Confocal Microscopy
Muscle Cells
Electron Microscopy
Fluorescence

Keywords

  • Anopheles
  • Cell architecture
  • Drosophila
  • Malaria
  • Salivary gland
  • Secretion

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi. / Wells, Michael B.; Andrew, Deborah J.

In: Parasites and Vectors, Vol. 8, No. 1, 617, 02.12.2015.

Research output: Contribution to journalArticle

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